GB/T 26625-2011Inspection of grain and oils - Determination of soybean isoflavone - High performance liquid chromatography (English PDF)
粮油检验 大豆异黄酮含量测定 高效液相色谱法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
June 16, 2011
Implementation date
November 1, 2011
Scope
GB/T 26625-2011 is the English-translated version of 粮油检验 大豆异黄酮含量测定 高效液相色谱法.
GB/T 26625-2011 is the Chinese standard "Inspection of grain and oils - Determination of soybean isoflavone - High performance liquid chromatography".
Its scope clause reads: This standard specifies the determination of soy isoflavones (daidzein, glycitein, genistein, daidzein, glycitein HPLC Aglycone, genistein principle) content, reagents and materials, instruments and equipment, reagent preparation and preservation, the steps, the results of calculation and said fine Density requirements.
This standard applies to soybeans, soy milk, tempeh determination of soybean isoflavone content. The detection limit of the standard test method was 2.5mg/kg. Its clauses include reagents and materials.
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Document preview — GB/T 26625-2011
National Standard of the People's Republic of China
- ICS
- 67.060
- Classification
- B 20
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 4 Reagents and materials
- 4.1 acetonitrile
- 4.9 Soybean glycosides (daidzin)
- 4.10 genistein (genistin)
- 4.11 daidzein
- 4.12 genistein
- 4.13 glycitein (glycitin)
- 4.14 glycitein aglycone (glycitein)
1 Scope
This standard specifies the determination of soy isoflavones (daidzein, glycitein, genistein, daidzein, glycitein HPLC Aglycone, genistein principle) content, reagents and materials, instruments and equipment, reagent preparation and preservation, the steps, the results of calculation and said fine
Density requirements.
This standard applies to soybeans, soy milk, tempeh determination of soybean isoflavone content.
The detection limit of the standard test method was 2.5mg/kg.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
Samples with methanol - aqueous ultrasonic oscillation extraction, the extract by centrifugation, concentration, volume, filtered, measured by high performance liquid chromatography, outside
Standard method.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade, water should meet GB/T 6682 stipulated a water.
4.1 acetonitrile
chromatography.
4.2
methanol.
4.3
acetic acid.
4.9 Soybean glycosides (daidzin)
purity not less than 98%.
4.10 genistein (genistin)
purity not less than 99%.
4.11 daidzein
purity not less than 98%.
4.12 genistein
purity not less than 98%.
4.13 glycitein (glycitin)
purity not less than 98%.
4.14 glycitein aglycone (glycitein)
purity not less than 98%.
4.15
Standard stock solution preparation.
4.15.1 soy isoflavones standard stock solution
namely, accurately weighed amount of daidzein, genistein, daidzein, genistein, soybeans
Flavin, glycitein aglycone standards, respectively with 60% methanol (4.5) formulated at a concentration of 1mg/mL standard stock solution. -18 °C to avoid
GB/T 15682-2008 | GB/T 22505-2008 | GB/T 22725-2008 | GB/T 21304-2026 | GB/T 21124-2026 | GB/T 26627.1-2011 | […]
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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