GB/T 25228-2010Inspection of grain and oils -- Determination of fumonisins in corn and its products by high liquid chromatography and fluorometer with immunoaffinity column cleanup (English PDF)

Foreword

The immunoaffinity column in this standard is purified by high performance liquid chromatography using the International Association of Analytical Chemists.

Methodology.2001.04 Determination of fumonisin in maize and maize flakes Immunoaffinity column Purification liquid chromatography "(Officialmethod for ease of use, this standard has the following modifications to AOACofficialmethod2001.04.

--- will be applied to the determination of the concentration range is modified to the detection limit, the deletion of the standard laboratory test data table A and Table B, increased Domestic laboratory test data;

To modify the requirements for immunoaffinity columns;

--- modify the concentration range of the standard curve;

--- will be fixed volume from.200µL to 400µL;

--- added a blank test;

--- simplifies the calculation of the results.

Appendix A, Appendix B and Appendix C of this standard are informative.

This standard is proposed by the National Grain Bureau.

This standard by the National Grain Standardization Technical Committee.

This standard is responsible for the drafting unit. State Food Bureau Science Research Institute.

This standard participates in the drafting unit. Beijing Zhongwei Weikang Technology Co., Ltd.

1 Scope

This standard specifies the use of immunoaffinity column purification of high performance liquid chromatography and immunoaffinity column purification fluorescence spectrophotometric determination of corn and its system the principle, reagents and materials, apparatus and equipment for the determination of fumonisin in products, procedures and results.

In this standard, immunoaffinity column purification and high performance liquid chromatography (HPLC) is suitable for the determination of fumonisin B1 and B2 in maize and maize products.

The immuno - spectrophotometric method for the determination of the total amount of fumonotoxin in maize and maize products.

The detection limit of immunoaffinity column purification and high performance liquid chromatography was 0.1mg/kg. The detection limit of immunoaffinity column was as follows.

0.5 mg/kg.

2 Normative reference documents

The terms of the following documents are hereby incorporated by reference into this standard. Whichever is the date of the reference file, which is followed by all (Excluding corrigenda) or revisions do not apply to this standard, however, encourage the parties to reach an agreement under this standard Whether you can use the latest version of these files. For undated references, the latest edition applies to this standard.

GB/T 6682 Analytical laboratory water specifications and test methods

3 Immunoaffinity Column Chromatography Purification by High Performance Liquid Chromatography