GB/T 25220-2010Inspection of grain and oils -- Determination of ochratoxin A in grains by high performance liquid chromatography and fluorometer (English PDF)

Foreword

Appendix A and Appendix B of this standard are informative.

This standard is proposed by the National Grain Bureau.

This standard by the National Grain Standardization Technical Committee.

This standard is responsible for the drafting unit. State Food Bureau Science Research Institute.

This standard participates in the drafting unit. Shanghai National Grain Quality Monitoring Center, Beijing Zhongwei Weikang Technology Co., Ltd., Jilin Province grain and oil hygiene Inspection station.

1 Scope

This standard specifies the use of high performance liquid chromatography and fluorescence spectrophotometric determination of ochratoxin A in food the principles, reagents and materials, Instruments and equipment, operating procedures and results.

This standard applies to the determination of ochratoxin A in wheat, maize and rice.

Immunoaffinity column purification and high performance liquid chromatography and immunoaffinity column purification fluorescence spectrophotometry detection limit of 1µg/kg, ion exchange the detection limit of high performance liquid chromatography was 0.8µg/kg.

2 Normative reference documents

The terms of the following documents are hereby incorporated by reference into this standard. Whichever is the date of the reference file, which is followed by all (Excluding corrigenda) or revisions do not apply to this standard, however, encourage the parties to reach an agreement under this standard Whether you can use the latest version of these files. For undated references, the latest edition applies to this standard.

GB/T 6682 Analytical laboratory water specifications and test methods

3 Immunoaffinity column purification by high performance liquid chromatography