GB/T 24829-2009Identification of Trichodorus species as virus vectors for import and export quarantine (English PDF)
毛刺线虫属（传毒种类）检疫鉴定方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 15, 2009
Implementation date
June 1, 2010
Scope
GB/T 24829-2009 is the English-translated version of 毛刺线虫属（传毒种类）检疫鉴定方法.
GB/T 24829-2009 is the Chinese standard "Identification of Trichodorus species as virus vectors for import and export quarantine". Its scope clause reads: This standard specifies the case of virus transmission glitch nematode species of quarantine and identification methods.
This standard applies to plants, and soil media glitch nematode species belong to mass poisoning of quarantine and identification. Its clauses include based on identification; apparatus and appliances; reagents.
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Document preview — GB/T 24829-2009
National Standard of the People's Republic of China
- ICS
- 65.020.01
- Classification
- B 16
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Identification 1 basis
- 5 Reagent
- 6 2 quarantine site
- 7 Laboratory test
- 8 morphological identification of
- 10 4 Sample Storage
- Appendix A (informative) virus transmission glitch nematode species basic information table
- Annex B (informative) nematode separation method
- Annex C (informative) nematode slide specimen preparation method
- Annex D (informative) four kinds of virus transmission glitch nematode dynamometers value Table
- Appendix E (normative) Identification of the genus burr polytomous keys (male)
- Annex F (informative) Identification of the genus burr polytomous keys (female)
- Annex G (informative) multi-manifold retrieval application reference table
- Appendix H (informative) four kinds of mass poisoning kinds of patterns
- References
1 Scope
This standard specifies the case of virus transmission glitch nematode species of quarantine and identification methods.
This standard applies to plants, and soil media glitch nematode species belong to mass poisoning of quarantine and identification.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. Where dated references, which subsequently
Some amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Study whether the latest versions of these documents. Anyone who does not dated reference documents, the latest versions apply to this standard.
SN/T 1157 export plant seedlings quarantine rules
SN/T 1158 bonsai plant quarantine entry and exit procedures
3 based on identification
Chinese name. Burr nematode genera.
English name. stubbyrootnematode.
1877], no side tail gland Gang (AdenophoreaLinstow, 1905), three spear head (TriplonchidaCobb, 1922), membrane Pia mesh (Diph- therophorinaCoomans & Loof, 1970), glitches superfamily [Trichodoridea (Thorne, 1935) Siddiqi, 1974], Section glitch
[Trichodoridae (Thorne, 1935) Siddiqi, 1974], burr subfamily (TrichodorinaeThorne, 1935).
Burr nematode genus has been found in 56 species of perennial herbaceous or woody roots migratory external parasitic nematodes. Not only directly feeding
Plant root cause plant roots chunky, swelling, increased lateral roots and cause the host to malnutrition, growth disorders and other hazards, more serious is known
Of which there are four kinds can spread the tobacco rattle virus (tobaccorattlevirus, TRV) and pea early-browning virus (peaearly-browningvi-
Glitch nematodes mainly through seedlings with roots, soil and media communication, its female and male is mainly based on morphological characteristics of species identification, host
Scope and geographical distribution of information for reference (see Appendix A).
4 apparatus and appliances
4.1 Instrument
Biological microscope (50-fold to 1000-fold), stereo microscope (more than 10 times, with the transmission source), electric incubator, pipettes (20µL ~ 200µL), centrifuge (2000r/min or more).
4.2 Appliances
Clock face pan or dish, cover slips, slides, scissors, a pick, alcohol lamp, filter paper or gauze nematode, 500µL plastic tubes, cell culture
Dish, airtight plastic boxes, funnel, funnel rack, latex tubes, sealing clamp, heating plate, sieve-like (500 mesh).
5 Reagents
4% formaldehyde, glycerol, nail polish, wax, solution I (96% ethanol. glycerol. = 20.1.79 distilled water), the solution II (% glycerol .96
Ethanol = 1.19).
GB/T 43165-2023 | GB/T 40135-2021 | GB/T 19495.4-2018 | GB 15569-2026 | GB/T 24831-2009 | GB/T 24830-2009 | […]
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 25 pages — is available in the English PDF.
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