GB/T 23180-2008Feed additive - 2%d-Biotin (English PDF)

1 Scope

GB/T 23180-2008 is the Chinese national standard on feed additive - 2%d-biotin, in the field of agriculture. The /T suffix marks it as a recommended standard: it is not compulsory by itself, but it becomes binding as soon as a contract, a tender or a customer specification calls it up - which in practice is how most foreign buyers meet it. It was issued on 31 December 2008 by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China; Standardization Administration of China, and has been in force since 1 May 2009. Classification: ICS 65.120, CCS B46. This page is published from the official record of the standard held by the Chinese standards administration: the identification, the dates, the classification and the issuing body are taken from there. The clause text, the tables and the numeric limits are in the document itself, which is delivered complete in English translation.

This Standard specifies the requirements, test methods, inspection rules and requirements for labeling, packaging, transportation and storage of feed additive 2% d- Biotin products. This Standard is applicable to feed additives containing 2% d-Biotin prepared by spraying method and dilution method with starch, dextrin or lactose as carriers. Molecular formula: C10H16N2O3S. Relative molecular mass: 244.31 (2005 international relative atomic mass). Structural formula:

2 Normative references

The provisions in following documents become the provisions of this Standard through reference in this Standard. For dated references, the subsequent amendments (excluding corrigendum) or revisions do not apply to this Standard, however, parties who reach an agreement based on this Standard are encouraged to study if the latest versions of these documents are applicable. For undated references, the latest edition of the referenced document applies.

GB/T 602, Chemical reagent - Preparations of stock standard solutions ( GB/T 602- 2002, ISO 6353-1:1982, NEQ)

GB/T 603, Chemical reagent - Preparations of reagent solutions for use in test methods ( GB/T 603-2002, ISO 6353-1:1982, NEQ)

GB/T 6435, Determination of moisture in feedstuffs (

GB/T 6435-2006, ISO Pipette 0.50mL of trifluoroacetic acid into a 1000mL volumetric flask. Use ultrapure water to set volume constant to the scale mark.

4.1.5 Extractant: trifluoroacetic acid solution (4.1.4) + acetonitrile (4.1.1) = 75 + 25.

4.1.6 d-Biotin reference substance: d-Biotin content >= 99.0%.

4.1.7 d-Biotin standard stock solution: Weigh about 100mg of (accurate to 0.00001g) biotin reference substance (4.1.6). Place in a 50mL volumetric flask. Use extractant (4.1.5) to dissolve. Dilute and set volume constant to the scale mark. Shake well. The standard stock solution contains 2.0mg of biotin per milliliter.

4.1.8 d-Biotin standard working solution: Accurately pipette 1.00mL of d-Biotin standard stock solution (4.1.7) into a 10mL volumetric flask. Use extractant (4.1.5) to dilute and set volume constant to the scale mark. Shake well. The standard working solution contains 200µg of biotin per milliliter.

4.2 Instruments and equipment Common laboratory equipment and:

4.2.1 Ultrasonic water bath.

4.2.2 Ultrapure water device.

4.2.3 High-performance liquid chromatograph, with a UV tunable wavelength detector (or diode matrix detector).

4.3 Identification test Take the specimen solution. Measure it by high performance liquid chromatography. The relative retention time of the main peak of the sample solution is consistent with the relative retention time of the main peak of the control solution.

4 Test methods...

5 Inspection rules

5.1 Exit-factory inspection The feed additive 2% d-Biotin shall be inspected by the quality supervision department of the manufacturer according to this Standard. All indicators specified in this Standard are exit-factory inspection items. Manufacturers shall ensure that all d-Biotin products meet the requirements specified in this Standard. Each batch of products can only leave the factory after passing the inspection.

5.2 Sampling method Clean, dry, airtight sample bottles are required for sampling. A label is attached to the bottle and marked with manufacturer name, product name, batch number, sampling date. When sampling, use a clean and suitable sampling tool to insert three-quarters of the depth of the material layer. Mix the samples taken thoroughly. Use quartering method to reduce. Each batch of samples is divided into two portions. The amount of each portion shall be three times the amount of the specimen required for inspection. Load into the sample bottles. One bottle is for inspection. The other bottle is sealed, stored for possible inspection.

5.3 Rules for determination If one of the indicators in the inspection results does not meet the requirements of this Standard, the sampling shall be doubled for re-inspection. If there is still one indicator in the re-inspection results that does not meet the requirements of this Standard, the entire batch of products shall be rejected.

6 Labeling, packaging, transportation and storage

6.1 Labeling According to GB 10648.

6.2 Packaging Products are accurately weighed and packed into aluminum foil bags or metal cans. Seal. Store in outer packaging containers. Store airtight.