GB/T 22101.1-2026Technical specification for the evaluation of resistance to diseases and insect pests in cotton - Part 1: Cotton bollworm (English PDF)

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part

1.Structure and Drafting Rules of Standardization Documents". Drafting. This document is Part 1 of GB/T 22101, "Technical Specification for Evaluation of Cotton Disease and Pest Resistance". GB/T 22101 has already published the following... part.

1.The Cotton Bollworm;

3.Pink Bollworm;

5.Verticillium Wilt. This document supersedes GB/T 22101.1-2008 "Technical Specification for Evaluation of Cotton Disease and Pest Resistance Part

1.Cotton Bollworm", and is consistent with GB/T 22101.1- Compared to.2008, aside from structural adjustments and editorial changes, the main technical changes are as follows:

---The scope has been changed (see Chapter 1, Chapter 1 of the.2008 edition);

---The "Terms and Definitions" section has been revised, adding the terms "genetically modified insect-resistant cotton," "susceptible bollworm-infested cotton strains," and "bollworm infestation reduction rate." The terms and definitions have been revised, removing "larval mortality rate," "newly hatched larvae," and "double dish," and changing the term "standard susceptible insect." Definition of "cotton variety" (see Chapter 3, Chapter 2 of the.2008 edition);

---The requirements related to "Bollworm in Test Cotton" have been revised (see 4.1, Chapter 3 of the.2008 edition);

---The selection settings for "Control Material" have been changed, and seed quality requirements have been added (see 4.2,

3.6 in the.2008 version);

---Added "Data Records" (see 4.3);

---The indoor insect resistance assessment method has been changed, removing the "double-plate collective insect feeding method" and the "separate single-head rearing method" as well as the "adjustment of assessment results". See Chapter 5,.2008 edition, sections 4.3, 4.4, and 4.5;

---The method for identifying field greenhouses has been changed. The revised method is divided into "Sowing and Field Management," "Insect Inoculation Methods," "Survey Records," and "Result Calculation." The results analysis and presentation consist of five parts (see Chapter 6, sections 5.2, 5.3, and

1 Scope

GB/T 22101.1-2026 is the Chinese national standard covering testing a cotton variety against the bollworm - the infestation method, the damage scoring and the classification of resistance, which for Chinese cotton mostly means confirming that the Bt trait is expressing as it should. It replaces GB/T 22101.1-2008 and has been in force since 1 October 2026, with Part 3 on pink bollworm. It was issued on 31 March 2026 and takes effect on 1 October 2026, replacing GB/T 22101.1-2008. The document is under the responsibility of the Ministry of Agriculture and Rural Affairs. This page is published from the official record of the 2026 edition; the clause text of a standard this recent is not yet in circulation, and the figures, limits and tables it contains are those of the document itself, delivered in full with the English translation.

This document defines the terminology and definitions for cotton bollworm resistance evaluation techniques, and specifies procedures for indoor resistance identification and field resistance testing. The content describes the verification method. This document applies to the indoor and field identification and evaluation of resistance to bollworm in genetically modified and non-genetically modified cotton.

4 Requirements

4.1 Test cotton bollworm Sensitive cotton bollworm strains were selected. Larvae were reared with artificial feed, and adults were fed a 5%-10% honey water solution. Indoor rearing conditions were as follows: Below. Temperature 26°C±2°C, photoperiod 14h.10h (light.dark), relative humidity 70%±10%. Artificial feed formulation and... The preparation method is shown in Appendix A.

4.2 Tested cotton varieties (lines) Tested cotton varieties (lines), standard insect-susceptible cotton varieties, and insect-resistant cotton varieties (locally cultivated transgenic insect-resistant cotton varieties). Seed quality. The quantity should meet the requirements of GB 4407.1.

5 Indoor Insect Resistance Identification

5.1 Sowing and Field Management Sow according to local conventional sowing time, method, and amount. The plot area should be no less than 30m2, with 3 replicates, and arranged in a randomized block design. Cotton seeds should not be coated or treated with any insecticides. During the bollworm outbreak period, pesticides that affect lepidopteran pests should not be sprayed. Other practices should follow local field regulations. Management is carried out in the room.

5.2 Inoculation Method During the peak occurrence periods of the second, third, and fourth generations of bollworm larvae in the field, 20 healthy cotton plants were randomly selected from each plot. Collect one fully unfolded leaf from the top or lateral fruiting branch of each cotton plant. Preserve in an icebox and promptly bring back to the laboratory for resistance testing. Insect identification. Select a suitable insect rearing container, place one cotton leaf inside, keep it moist, and ensure the cotton leaf does not dry out. Inoculate each cotton leaf with one-day-old larvae. Five insects. After inoculating the insects, seal the insect rearing container to prevent the cotton bollworms from escaping, and place it in an insect rearing room or incubator under the same environmental conditions as in 4.1.

5.3 Survey Records On the 5th day after inoculation, check the mortality of the larvae. Visually inspect and gently touch the larvae with the tip of a paintbrush; record those that react as live larvae, and those that do not react as dead larvae. Then record as death (according to Table B.1 in Appendix B). If the mortality rate of larvae in standard susceptible cotton varieties is <=20%, the mortality rate of larvae in the tested cotton varieties (lines) is recorded as death. The insect mortality rate needs to be corrected; if the mortality rate of larvae in standard susceptible cotton varieties is >20%, the cause needs to be analyzed and the experiment repeated.

6 Field insect resistance test

6.1 Sowing and Field Management The tested cotton varieties (lines) were covered with 40-mesh (0.425mm) nylon netting. The netting height was 1.8m and the area was no more than 50m2. The tested cotton varieties (lines) were planted in single rows within the cages, with 30 plants per row, without duplication. Protective rows were set up around the perimeter of the cages, and the cultivation method within the cages was the same as for the larger varieties. Field. 10-15 days before releasing the adults, apply a broad-spectrum chemical pesticide to control the pests once, preventing interference from other insects in the cage. Other treatments should be carried out as needed. Routine field management is carried out.

6.2 Inoculation Method During the cotton boll-forming stage, release adult bollworms on a non-rainy day. Select healthy adult bollworms that emerged at the same time, arranged according to the female-to-male ratio. They were kept in a

1.1 ratio and allowed to mate freely in an indoor insect rearing cage, supplemented with 10% honey water for nutrition. Three days after mating, selection was made based on activity level. Strong adult insects were released into the cages described in 6.1.Four pairs were released per 10 m^2.

6.3 Survey Records Three days after the release of adult bollworms, the egg count should be assessed. The number of eggs per 100 plants should be greater than 300; otherwise, the adult release rate should be increased. (Inoculation) 10-15 days later, the number of damaged and healthy buds and bolls on each cotton plant of the tested cotton variety (line) was investigated (according to Table B.2), and the bud and boll damage rate was calculated. (d) Leiling victim reduction rate (z).