GB/T 21101-2025Determination of porcine-derived material in feeds (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

This document replaces GB/T 21101-2007 "Qualitative detection method of porcine-derived ingredients in animal feed - PCR method" and Compared with GB/T 21101-2007, in addition to structural adjustments and editorial changes, the main technical changes are as follows.

a) The scope of application and detection limit have been changed (see Chapter 1, Chapter 1 of the 2007 edition);

b) The term and definition of “polymerase chain reaction” have been deleted (see 3.1 of the 2007 edition);

c) Added abbreviations (see Chapter 4);

d) Added real-time fluorescent polymerase chain reaction method (see Chapter 5);

e) The sample has been changed (see 6.4, Chapter 7 of the 2007 edition);

f) The result determination and presentation have been changed (see 6.6, Chapter 9 of the 2007 edition);

g) The laboratory pollution prevention and control measures have been modified (see Chapter 7, Chapter 10 of the 2007 edition);

h) Waste disposal has been modified (see Chapter 8, Chapter 11 of the 2007 edition).

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76).

This document was drafted by: Sichuan Well Testing Technology Co., Ltd., Chengdu Customs Technology Center, Chengdu Agricultural Science and Technology Vocational College, Qinghai Vocational College, Island Customs Technical Center, Shenzhen Customs Animal and Plant Inspection and Quarantine Technical Center, Dalian Customs Technical Center.

The main drafters of this document are: Yang Miao, Yang Xinyi, Zhang Jing, Zhang Fengping, Lin Hua, Xiao Xian, Li Jianzhen, Lu Jiawen, Yu Xinghong, Zhang Yanhong, Lu Lihua, Lei Hui, Sun Tao, Ruan Zhouxi, Chen Xi, An Wei, Zheng Jing, Chen Yingqi, Xue Changhua, Zhang Qian, and Chen Runjiang.

The previous versions of this document and the documents it replaces are as follows.

— First published in 2007 as GB/T 21101-2007;

— This is the first revision.

1 Scope

This document describes the real-time fluorescent polymerase chain reaction (RT-PCR) and polymerase chain reaction (PCR) methods for the determination of porcine-derived ingredients in feed.

(PCR) qualitative detection method.

The real-time fluorescence PCR method in this document is applicable to compound feed, concentrated feed, concentrate supplement, compound premix feed, feed raw materials and mixed Qualitative detection of porcine-derived ingredients in compound feed additives. PCR method is suitable for the qualitative detection of porcine-derived ingredients in animal-derived feed raw materials. Detection.

The detection limit for this document is 0.1%.

2 Normative references

GB/T 6682

GB/T 19495.3-2004

GB/T 20195

GB/T 27403-2008

3 Terms and Definitions

There are no terms or definitions that require definition in this document.

4 Abbreviations

The following abbreviations apply to this document. bp. base pair Ct. cycle threshold

5 Real-time fluorescence PCR