GB/T 20944.3-2008Textiles - Evaluation for antibacterial activity - Part 3: Shake flask method (English PDF)

Foreword

GB/T 20944 "Textiles - Evaluation for antibacterial activity" consists of three parts.

- Part 1: Agar diffusion plate method;

- Part 2: Absorption method;

- Part 3: Shake flask method.

This Part is Part 3 of GB/T 20944.

1 Scope

This Part of GB/T 20944 specifies quantitative test and evaluation method for determining the antibacterial properties of textiles by the shake flask method.

This Part is applicable to various textile products such as down, fiber, yarn, fabric, and products with special shapes, especially applicable to non-dissolvable antibacterial textile products.

This Part does not involve the evaluation of the safety of antibacterial products.

2 Normative references

The provisions in following documents become the provisions of this Part of GB/T 20944 through reference in this Standard. For dated references, the subsequent amendments (excluding corrigendum) or revisions do not apply to this Standard, however, parties who reach an agreement based on this Standard are encouraged to study if the latest versions of these documents are applicable. For undated references, the latest edition of the referenced document applies.

GB/T 12490, Textiles - Tests for color fastness - Color fastness to domestic and commercial laundering (GB/T 12490-2007, ISO 105-C06.1994, MOD)

3 Terms and definitions

For the purposes of this document, the following terms and definitions apply.

4 Safety precautions

Safety instructions. The test bacteria used in this test can cause human infection and disease.

5 Principle

Put the sample and the control fabric into an Erlenmeyer flask that is filled with a certain concentration of test bacteria liquid.

6 Test equipment

6.1 Spectrophotometer. The detection wavelength is 475nm or 660nm, suitable for testing the concentration of test bacteria.

6.2 Constant temperature incubator. temperature control accuracy is ±1°C

6.7 Autoclave. The temperature can be kept at 121°C and the pressure can be kept at 103kPa.

6.8 Erlenmeyer flask with stopper. The capacity is 250mL

6.3 Water bath. The temperature can be maintained at 46°C±2°C

7 Medium and reagents

The reagents used in the test shall be analytically pure or suitable for microbiological tests. The test water shall be pure water, such as distilled water.

7.4 0.03mol/L PBS (phosphate) buffer Disodium phosphate 2.84g

8 Test strain

9 Preparation of test bacteria

10 Preparation of sample

11 Test operation

11.3 "0" contact time sampling Use a pipette to draw 1mL ± 0.1mL of solution from each of the 6 flasks prepared with a contact time of "0". Transfer to a test tube containing 9mL ± 0.1mL of 0.03 mol/L PBS buffer (7.4). Mix well. Use 10 times dilution method to make another dilution. Mix well. Pipette 1mL±0.1mL into a sterilized petri dish, pour about 15mL of nutrient agar medium (7.2) or Sabouraud agar medium (7.3).