GB/T 19495.5-2018Detection of genetically modified organisms and derived products—Quantitative real-time polymerase chain reaction (PCR) methods (English PDF)

Foreword

GB/T 19495 "GMO Testing" is divided into the following sections.

---GB/T 19495.1 General requirements and definitions for the detection of genetically modified products;

---GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products;

---GB/T 19495.3 Detection of nucleic acid extraction and purification methods for transgenic products;

---GB/T 19495.4 Transgenic product detection real-time fluorescence qualitative polymerase chain reaction (PCR) detection method;

---GB/T 19495.5 Transgenic product detection real-time fluorescence quantitative polymerase chain reaction (PCR) detection method;

---GB/T 19495.6 Genetically modified product detection gene chip detection method;

---GB/T 19495.7 GM product testing sampling and sample preparation methods;

---GB/T 19495.8 Detection method for protein detection of genetically modified products;

---GB/T 19495.9 Detection method of liquid product chip for detection of plant products by genetically modified products.

This part is the fifth part of GB/T 19495.

This part is drafted in accordance with the rules given in GB/T 1.1-2009.

This part replaces GB/T 19495.5-2004 "Quantitative PCR detection method for detection of nucleic acid in genetically modified products". And GB/T 19495.5- Compared with.2004, the main technical changes except editorial changes are as follows.

--- Revised the scope of application of the standard;

--- Added the ability to quantitatively detect transgenic plant lines in samples based on matrix reference materials and plasmid standard molecules, respectively. Procedure

--- Increased the copy number of transgenic plant lines such as soybean, corn, canola, cotton, rice, potato, sugar beet and papaya Quantitative detection method.

1 Scope

This part of GB/T 19495 specifies soybeans, corn, rapeseed, rice (rice), cotton, potatoes, beets, papaya and other plants and A real-time fluorescent PCR quantitative detection method for the content of transgenic lines in products.

This section applies to quantitative detection methods for hundreds of copies of transgenic lines in the above plants and their products.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products

GB/T 19495.3 Transgenic product detection nucleic acid extraction and purification method

GB/T 19495.7 GM product testing sampling and sample preparation method

GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing

JJF1059.1 Measurement Uncertainty Evaluation and Representation

SN/T 4562 Transgenic Testing Laboratory Measurement Uncertainty Evaluation Guide

3 Terms, definitions and abbreviations