GB/T 17528-2009Determination of pipeline content -- Method using HPLC (English PDF)

Foreword

This revised standard adopts ISO 11027:1993 "pepper and pepper oleoresin pepper alkali content by HPLC" this standard and ISO 11027:1993 main differences are as follows.

--- The ISO 11027:1993 the name changed to Determinationofpiperinecontent-Methodusinghigh-per-

--- Increasing the detection limit;

--- The ISO 11027:1993 in a concentration range of 6.2 piperine standard solution "0.05g/L ~ 0.2g/L" changed to "0.4mg/L ~ 2.0mg/L ";

--- The ISO 11027:1993 in 4.3 mobile phase "of acetonitrile and 1% acetic acid (48 + 52)" to "methanol - water (77 + 23)";

--- Cancellation ISO 11027:1993 10.1 10.2 formulas and calculations factor, because no formula to calculate the revised factor.

This standard replaces GB/T 17528-1998 "Determination pepper alkali content spectrophotometry." this standard and GB/T 17528-1998 compared to major differences are.

--- The spectrophotometric method to the High Performance Liquid Chromatography.

Appendix A of this standard is an informative annex.

The standard proposed by the People's Republic of China Ministry of Agriculture.

This standard by the national spice Standardization Technical Committee.

This standard was drafted. the Ministry of Agriculture Food Quality Supervision and Testing Center (Zhanjiang).

1 Scope

This standard specifies the method for determining pepper alkali content by high performance liquid chromatography.

This standard applies to black, white and black pepper, white pepper and pepper oleoresin extraction product Content Determination.

The standard method detection limit of 0.008g/100g.

Principle 2

The sample was extracted with ethanol piperine detected by HPLC UV detector, external standard.

3 Reagents

Unless otherwise indicated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity.

3.1 Ethanol (CH3CH2OH). 95% (mass fraction).

3.2 methanol (CH3OH). chromatography.

3.3 piperine standard substance. purity >=98%.

Piperine 3.4 Standard solution. Accurately weigh (10 ± 0.1) mg piperine standard brown substance in 10mL beaker, ethanol (3.1) was dissolved Solution, transferred to a 10mL brown volumetric flask with ethanol (3.1) to the mark, the concentration of the solution was 1000mg/L (when used with temporary system).

3.5 piperine standard working solution. lessons 50µL piperine standard solution (3.4) with a micropipette placed in 25mL brown volumetric flask, Ethanol (3.1) diluted to the mark, the concentration of the solution is 2.00mg/L.

4 Instrument

4.1 High performance liquid chromatograph. equipped with a UV detector.

4.2 Organization mashed machines.

4.3 Sample screens. a pore size of 500µm.

4.4 brown round bottom flask. 100mL, supporting reflux condenser apparatus.

4.5 brown volumetric flask. 10mL, 25mL, 100mL.

5 Sample Preparation

6 Step, Analysis

Note. Because piperine solution is unstable, easily decomposed in light, dark operation is necessary. Operation with black paper or aluminum foil and the flask flask wrapped up, and Determination as soon as possible.