GB/T 14926.56-2008Laboratory animal - Method for examination of rabies virus (English PDF)

3 Main reagents and materials

4 Test methods

5 Assessment of the results

6 Interpretation of the results

7 Reporting of the results

Foreword

GB/T 14926, Laboratory animal, consists of 54 parts, covering test methods for different microorganisms and viruses.

From the date of its implementation, this part replaces GB/T 14926.56-2001, Laboratory animal - Method for examination of rabies virus.

Compared with GB/T 14926.56-2001, the main technical differences of this part are as follows:

a) the content of "2 Normative references" of the original standard has been deleted;

b) the entire content of "3 Principle" of the original standard has been replaced by: "Purified rabies virus antigen is pre-coated on a microplate so that the immune reaction takes place on a solid-phase carrier. When rabies virus antibody is present in the serum under test, it forms an antigen-antibody complex with the antigen on the well wall, which then reacts with an enzyme-labelled anti-canine IgG conjugate; finally, qualitative detection is performed by measuring the product formed by the enzyme-catalysed substrate reaction";

c) all reagents, methods and result criteria relating to the haemagglutination inhibition test in the original standard have been deleted;

d) all content relating to the enzyme-linked immunosorbent assay in the original standard has been revised, including reagents, methods and result criteria;

e) "6.3 Qualification rate of immune antibody in basic-grade dog populations: immune antibody qualification rate = (number of antibody-positive animals tested / total number of animals tested) × 100 %. A basic-grade dog population whose immune antibody qualification rate reaches 70 % or above may be judged as immunologically qualified" has been added to "6 Determination of results" of the original standard.

This part was proposed by and is under the jurisdiction of the National Technical Committee for Standardization of Laboratory Animals.

This part was drafted by the National Technical Committee for Standardization of Laboratory Animals.

Main drafters of this part: Tian Kegong, Chen Xizhao, Cao Zhen, Wang Chuanbin, He Xiumin, Tang Hanwen.

Previous editions of the standard replaced by this part:

- GB/T 14926.56-2001.