GB 1886.351-2021National Food Safety Standard - Food Additives - alpha-cyclodextrin (English PDF)

2.1 Molecular formula

(C6H10O5)6

2.2 Relative molecular mass

972.85 (according to 2018 international relative atomic mass)

3.1 Sensory requirements

Sensory requirements shall meet the requirements of Table 1.

3.2 Physical and chemical indicators

The physical and chemical indicators should meet the requirements of Table 2.

A.1 General

The reagents and water used in this Standard refer to analytically-pure reagents and grade 3 water specified in GB/T 6682 when other requirements are not indicated. The standard solutions used in the test, standard solutions for impurity determination, preparations and products, unless other requirements are specified, are prepared in accordance with the provisions of GB/T 601, GB/T 602 and GB/T 603. The solution used in the test refers to an aqueous solution when it is not specified which solvent is used to prepare it.

A.2 Identification test

A.2.1 Specific rotation test

Weigh 1.00g of specimen and dissolve it in 100mL of water. Determine according to the method specified in GB/T 613. The specific rotation shall be +145°~+151°.

A.2.2 Chromatography test

In the alpha-cyclodextrin content determination test, the retention time of the main peak of the test solution chromatogram shall be consistent with the retention time of the main peak of the standard solution chromatogram.

A.3 Determination of alpha-cyclodextrin content (on a dry basis)

A.3.1 Reagents and materials

A.3.1.1 Methanol: Chromatographically pure.

A.3.1.2 Water: Grade 1 water specified in GB/T 6682

A.3.1.3 alpha-cyclodextrin standard product: Purity >=98.0%.

A.3.2 Instruments and equipment

High performance liquid chromatograph: Equipped with a refractive index detector.

A.3.3 Reference chromatographic conditions

A.3.3.1 Chromatographic column

C18, C8, phenyl column and other reverse chromatographic columns (5µm, 150mm×4.6mm).