GB 1886.345-2021National food safety standard - Food additives - Mulberry red (English PDF)

2.1 Molecular Formula

Cyanidin-3-glucoside: [C21H21O12]+ X- Cyanidin-3-rutinoside: [C27H31O15]+ X- X- is the counter ion.

2.2 Structural Formula

Cyanidin-3-glucoside: R1 = glucosyl Cyanidin-3-rutinoside: R1 = rutinosyl

3.1 Sensory Requirements

The sensory requirements shall comply with the requirements of Table 1.

A.1 General Rules

When other requirements are not specified, the reagents and water used in this Standard refer to analytically pure reagents and Grade-3 water specified in GB/T 6682.

When other requirements are not specified, the standard solutions, preparations and products used in tests shall be prepared in accordance with the stipulations of GB/T 601, GB/T 602 and GB/T 603. When it is not specified which solvent is used for preparation, the solutions used in tests refer to aqueous solutions.

A.2 Identification Test

A.2.1 Reagents and materials

A.2.1.1 Trifluoroacetic acid: chromatographically pure.

A.2.1.2 Acetonitrile: chromatographically pure.

A.2.1.3 Sodium hydroxide solution: 2 mol/L.

A.2.1.4 Hydrochloric acid solution: 2 mol/L.

A.2.1.5 Disodium hydrogen phosphate solution: 0.2 mol/L. Accurately weigh-take 71.64 g of disodium hydrogen phosphate (Na2HPO4·12H2O); use water to dissolve it and dilute to a constant volume of 1,000 mL.

A.2.1.6 Citric acid solution: 0.1 mol/L. Accurately weigh-take 21.01 g of citric acid (C6H8O7·H2O); use water to dissolve it and dilute to a constant volume of 1,000 mL.

A.2.1.7 Citric acid - disodium hydrogen phosphate buffer solution: pH 3.0. Measure-take 41 mL of 0.2 mol/L disodium hydrogen phosphate solution and 159 mL of 0.1 mol/L citric acid solution; mix them up.

A.2.1.8 Trifluoroacetic acid solution: 0.1% (volume fraction).

A.2.1.9 Extraction solvent: trifluoroacetic acid solution + acetonitrile (75 + 25).

A.2.1.10 Cyanidin-3-glucoside (kuromanin chloride) reference substance: CAS: 7084-24-4, purity >= 98%.

volume of 100 mL; filter them through a 0.45 µm filter membrane.

A.2.3.3.3 Reference chromatographic conditions

A.2.3.3.3.1 Chromatographic column: C18 reversed phase column (phi3 mm × 150 mm, 3.5 µm), or other equivalent chromatographic columns.

A.2.3.3.3.2 Mobile phase A: trifluoroacetic acid solution (A.2.1.8).

A.2.3.3.3.3 Mobile phase B: acetonitrile (A.2.1.2).

A.2.3.3.3.4 Flow rate: 0.4 mL/min.

A.2.3.3.3.5 Detection wavelength: 520 nm

A.2.3.3.3.6 Injection volume: 2 µL

A.2.3.3.3.7 Column temperature: 35 °C

A.2.3.3.3.8 Isocratic elution conditions: mobile phase A + mobile phase B = 89 + 11

A.2.4 Analytical procedures

Under the reference chromatographic conditions of A.2.3.3.3, respectively inject the reference solution and specimen solution of cyanidin-3-glucoside (kuromanin chloride) and cyanidin-3-rutinoside.

A.2.5 Result determination

The chromatogram of the specimen solution shall manifest two obvious main peaks, and the retention time of the two main peaks shall be consistent with the retention time of the reference substance of cyanidin-3-glucoside (kuromanin chloride) and cyanidin-3-rutinoside.

A.3 Determination of Color Value

A.3.1 Reagent and material

Citric acid - disodium hydrogen phosphate buffer solution: pH 3.0. Same as A.2.1.7.

A.3.2 Instruments and equipment

A.3.2.1 UV spectrophotometer.

A.3.2.2 Cuvette: 1 cm.

A.3.3 Analytical procedures

Inject the specimen solution (A.2.3.2) into a 1 cm cuvette. Take the citric acid - disodium hydrogen phosphate buffer solution (A.2.1.7) as the blank; use the spectrophotometer

the distillation method in GB 5009.34, expressed in (g/kg);

1,000---the unit conversion factor.

---the color value of the specimen being tested.

The arithmetic mean value of parallel determination results shall prevail in the test result. The absolute difference between two independent determination results obtained under repeatability conditions shall not exceed 10% of the arithmetic mean value.

A.5 pH Determination

A.5.1 Instrument and equipment

pH meter.

A.5.2 Determination method

Weigh-take 1 g of specimen, accurate to 0.01 g. Completely dissolve it in distilled water and dilute to a constant volume of 100 mL. Use the pH meter to determine its pH value.

The arithmetic mean value of parallel determination results shall prevail in the test result. The absolute difference between two independent determination results obtained under repeatability conditions shall not exceed 0.1 pH.

A.6 Determination of Burning Residue

A.6.1 Instrument and equipment

A.6.1.1 Crucible.

A.6.1.2 High-temperature furnace.

A.6.1.3 Desiccator.

A.6.2 Determination method

Weigh-take 3 g of specimen, accurate to 0.001 g. Place it in a crucible that has reached a constant mass at 800 °C ± 25 °C. Firstly, slowly carbonize it on an electric furnace (at about 300 °C), then, transfer it into a high-temperature furnace at 800 °C ± 25 °C to burn to a constant mass.

A.6.3 Result calculation

The mass fraction w1 of the burning residue shall be calculated in accordance with Formula (A.3).