GB 1886.347-2021National food safety standard - Food additives -4-amino-5,6-dimethylthieno(2,3-d)pyrimidin-2(1H)-one hydrochloride (English PDF)

1 Scope

This standard is applicable to food additives, 4-amino-5,6-dimethylthieno(2,3-d)pyrimidin-2(1H)-one hydrochloride, which are produced through chemical reaction, by the raw materials of malononitrile, 3-mercapto-2-butanone, etc.

2 Chemical name, molecular formula, structural

formula, relative molecular mass

3 Technical requirements

Appendix A Determination of the content of 4-amino-5,6-dimethylthieno(2,3-

d)pyrimidin-2(1H)-one hydrochloride A.1 Reagents and materials A.1.1 Trifluoroacetic acid: Purity >= 98%.

A.1.1.1 The 0.1% trifluoroacetic acid solution: Pipette 1.00 mL of trifluoroacetic acid (A.1.1). Add it to a 1000 mL volumetric flask. Add water (A.1.3) to dilute it to the mark. Shake well.

A.1.1.2 The 0.05% trifluoroacetic acid solution: Pipette 0.50 mL of trifluoroacetic acid (A.1.1). Add it to a 1000 mL volumetric flask. Add water (A.1.3) to dilute it to the mark. Shake well.

A.1.2 Acetonitrile: Chromatographically pure.

A.1.3 Water: Grade 1 water as specified in GB/T 6682.

A.1.4 Control product of 4-amino-5,6-dimethylthieno(2,3-d)pyrimidin-2(1H)-one hydrochloride: Purity >= 99.0%.

A.2 Instrument and equipment Ultra-high performance liquid chromatograph: Equipped with a diode array detector.

A.3 Reference chromatographic conditions A.3.1 Chromatographic column: C18 liquid chromatographic column, which has a length of 100 mm, an inner diameter of 4.6 mm, a particle size of 3.5 um; OR other equivalent chromatographic column.

A.3.2 Mobile phase: Composed of 0.1% trifluoroacetic acid solution (A.1.1.1)

and acetonitrile (A.1.2), in a volume ratio of 95:5.

A.3.3 Column temperature: 30 °C.

A.3.4 Flow rate: 1.0 mL/min.

A.3.5 Injection volume: 1 uL.

A.3.6 Detection wavelength: 245 nm.