YY/T 1416.6-2021Test method for additive in single-use containers for human venous blood specimen collection - Part 6: Imidazolidinyl urea (English PDF)

Foreword

YY/T 1416 "Method for Determination of Additive Dose in Disposable Human Venous Blood Sample Collection Containers", which consists of the following parts.

---Part 1: Ethylenediaminetetraacetic acid (EDTA) salt;

---Part 2: Sodium Citrate;

---Part 3: Heparin;

---Part 4: Fluoride;

---Part 5: Glycine;

---Part 6: Imidazolidinyl urea; This part is part 6 of YY/T 1416.

This section was drafted in accordance with the rules given in GB/T 1.1-2009.

Introduction

Disposable human venous blood sample collection container, also known as. blood collection container or blood collection tube, matched with disposable venous blood sample collection needle Used to collect venous blood samples for clinical testing. Collection containers containing different additives or add-ons have different uses, free deoxygenation The ribonucleic acid (DNA) preservation tube is a special-purpose collection container for non-invasive prenatal screening, early tumor screening and other clinical test samples Collection and storage of books. The storage quality of free DNA plays an important role in the test results. In the process of transportation and storage, it is necessary to prevent plasma The nuclease in the nuclease degrades free DNA, but also avoids the release of genomic DNA in blood nucleated cells. Therefore, save the tube by adding Nuclease inhibitors and nucleated cell protective agents are necessary. As a preservative, imidazolidinyl urea is mainly used to inhibit the growth of microorganisms.

Propagation and stabilization of nucleated blood cells, thereby preventing the release of cellular genomic DNA, inhibiting the nuclease-mediated degradation of cell-free DNA, and helping to prevent cell-free DNA from nuclease-mediated degradation. The overall stability of cell DNA.

This section gives the spectrophotometric method for the determination of the amount of imidazolidinyl urea in a single collection container. Other measurements can also be used. The method is determined, but methodological confirmation is required.

1 Scope

This part of YY/T 1416 provides a single-use human venous blood sample collection container (collection) for the determination of imidazolidinyl urea in additives.

The test method of the imidazolidinyl urea additive in the container). This section applies to collection containers containing imidazolidinyl urea additives.

Note. For diazoalkyl urea additives, because the detection principle is the same, you can refer to the method given in this section.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this document. For undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

3 Acetylacetone colorimetry