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YY/T 1190-2010Lactose bile salt fermentation medium (English PDF)

乳糖胆盐发酵培养基

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Issued by

NMPA

Level / Type

Industry · Recommended

Issue date

December 27, 2010

Implementation date

June 1, 2012

Scope

YY/T 1190-2010 is the English-translated version of 乳糖胆盐发酵培养基.

YY/T 1190-2010 is the Chinese pharmaceutical industry standard for the lactose bile salt fermentation medium, used to detect coliforms by their fermentation of lactose with gas.

It specifies the quality requirements, the test methods, the instructions for use, the marking and labelling and the rules for packaging, transport and storage, covering the appearance and the pH of the prepared medium, its selectivity and the growth and the gas production of the control strains. Issued on 27 December 2010, it applies from 1 June 2012.

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Document preview — YY/T 1190-2010

National Standard of the People's Republic of China

ICS
11.100
Classification
C 44

Issued by: National Medical Products Administration of the People's Republic of China

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Terms and Definitions
  • 4 Medium formula

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

1 Scope

This standard defines the quality bile lactose fermentation medium requirements, test methods, instructions for use, marking, labeling and packaging, transportation, Storage.

This standard applies to the E. coli lactose bile lactose fermentation test fermentation medium.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 191 Packaging - Pictorial signs JJF 1070-2000 prepackaged goods measured net content inspection rules People's Republic of China Pharmacopoeia

3 Terms and Definitions

The following terms and definitions apply to this document.

3.1 Medium culture

Made by hand with the method specifically for microbial culture, separation, mixing nutrient products to identify, research and preservation used.

3.2 Quality control strains

Usually it refers to the medium used for quality control and performance measurement of microorganisms.

3.3 Colony forming units

colonyformingunit, CFU When the culture of viable counts by a plurality of cells or single cells gathered into groups on solid media growth and reproduction of colonies formed to The expression of the number of viable cells.

4 Medium formula

Unit. g/L Peptone 20.0 Lactose 10.0 Pig bile (bile salt or cattle, sheep bile) 5.0 Bromocresol purple 0.01

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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