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YY/T 1188-2010EMB agar medium (English PDF)

曙红亚甲蓝琼脂培养基

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Issued by

NMPA

Level / Type

Industry · Recommended

Issue date

December 27, 2010

Implementation date

June 1, 2012

Scope

YY/T 1188-2010 is the English-translated version of 曙红亚甲蓝琼脂培养基.

YY/T 1188-2010 is the Chinese pharmaceutical industry standard for the eosin methylene blue agar medium, the EMB medium on which enteric bacteria are isolated and differentiated.

It specifies the quality requirements, the test methods, the instructions for use, the marking and labelling and the rules for packaging, transport and storage, covering the appearance and the pH of the prepared medium, its selectivity and the growth and the colony appearance of the control strains. Issued on 27 December 2010, it applies from 1 June 2012.

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Document preview — YY/T 1188-2010

National Standard of the People's Republic of China

ICS
11.100
Classification
C 44

Issued by: National Medical Products Administration of the People's Republic of China

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Terms and Definitions
  • 4 Medium formula

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

This standard is a reference to "People's Republic of China Pharmacopoeia", GB/T 4789.28-2003 "Microbiological examination of food hygiene staining, Media and reagents ", WS/T 232-2002" commercial quality inspection of microbiological culture media "and the US National Committee for Clinical Laboratory Standards Members will (NCCLS) criteria, based on the combination of China's national conditions and developed the actual situation and requirements.

1 Scope

This standard defines the quality eosin methylene blue agar medium requirements, test methods, instructions for use, marking, labeling and packaging, transportation, Storage.

This standard applies to eosin methylene blue agar.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 191 Packaging - Pictorial signs People's Republic of China Pharmacopoeia JJF 1070-2000 prepackaged goods measured net content inspection rules

3 Terms and Definitions

The following terms and definitions apply to this document.

3.1 Medium culture

Made by hand with the method specifically for microbial culture, separation, mixing nutrient products to identify, research and preservation used.

3.2 Quality control strains

Usually it refers to the medium used for quality control and performance measurement of microorganisms.

3.3 Colony forming units

colonyformingunit, CFU When the culture of viable counts by a plurality of cells or single cells gathered into groups on solid media growth and reproduction of colonies formed to The expression of the number of viable cells.

4 Medium formula

Unit. g/L Peptone 10.0 Lactose 10.0 Dipotassium hydrogen phosphate 2.0 Eosin 0.4 Methylene blue 0.065 Agar 1) 13.0 to 17.0

1) content can be adjusted according to the agar gel strength of the finished product.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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