YC/T 159-2019Tobacco and tobacco products - Determination of water soluble sugars - Continuous flow method (English PDF)

Foreword

YC/T 405 "Tobacco and tobacco products – Determination of multi-pesticide

residues" is divided into five parts.

- Part 1. High performance liquid chromatography - tandem mass

spectrometry method;

- Part 2. Determination of organochlorine and pyrethroids pesticide – Gas

chromatographic method;

- Part 3. Gas chromatography - Mass spectrometry method and gas

chromatographic methods;

- Part 4. Determination of dithiocarbamate pesticide residues – Gas

chromatography-mass spectrometry method;

- Part 5. Determination of maleic hydrazide residue – High performance liquid

chromatography method.

This is Part 5 of YC/T 405.

This Part was drafted in accordance with the rules specified in GB/T 1.1-2009

AND GB/T 20001.4-2001.

Please note that some contents of this document may involve patents. The

issuing authority of this document does not undertake any responsibility of

identifying these patents.

This Part was proposed by the State Tobacco Monopoly Bureau.

This Part shall be under the jurisdiction of the National Tobacco Standardization

Technical Committee - Cigarette Institute Branch (SAC/TC 144/SC 1).

This drafting organizations of this Part. China National Tobacco Corporation -

Zhengzhou Tobacco Research Institute, National Tobacco Quality Supervision

and Inspection Center, China Tobacco Standardization Research Center,

Hongta Tobacco (Group) Co., Ltd., China Tobacco Hubei Industry Corporation.

1 Scope

This Part of YC/T 405 specifies the high performance liquid chromatography

method for the determination of maleic hydrazide residue in tobacco and

tobacco products.

This Part applies to the determination of maleic hydrazide pesticide residues in

tobacco and tobacco products. As for this method, the detection limit is

0.66mg/kg AND the quantitative limit is 2.18mg/kg.

2 Normative references

The following documents are essential to the application of this document. For

the dated documents, only the versions with the dates indicated are applicable

to this document; for the undated documents, only the latest version (including

all the amendments) are applicable to this standard.

GB/T 6682 Water for analytical laboratory use – Specification and test

methods

YC/T 31 Tobacco and tobacco products – Preparation of test sample and

determination of water content – Oven method

3 Principle

The crushed sample in the hydrochloric acid solution is heated for reflux and

extraction, then the extract is purified in C18 SPE column AND eluted by sodium

hydroxide solution, AND the elution is detected by the liquid chromatographic

UV detector.

4 Reagents and materials

6 Sample preparation

In accordance with YC/T 31, PREPARE sample and MEASURE the sample's

moisture content.

7 Operation steps