SN/T 4624.6-2016Methods for examination of import microbial blends in the environmental protection--Part 6: Staphylococcus aureus (English PDF)

Foreword

SN/T 4624 "Environmental Use of Microbial Agents for Environmental Protection" is divided into 17 parts.

--- Part 1. Bacillus licheniformis;

--- Part 2. Bacillus pumilus;

--- Part 3. Bacillus megaterium;

--- Part 4. Thiobacillus ferrooxidans;

--- Part 5. Beta hemolytic streptococci;

--- Part 6. Staphylococcus aureus;

--- Part 7. Salmonella;

--- Part 8. Shigella;

--- Part 9. Escherichia coli;

--- Part 10. Paecilomyces lilacinus;

--- Part 11. Elegant Chickwort;

--- Part 12. Trichoderma harzianum

--- Part 13. Phanerochaete chrysosporium;

--- Part 14. Aspergillus coli;

--- Part 15. Bacillus amyloliquefaciens;

--- Part 16. Pseudomonas alkaloids;

--- Part 17. Pseudomonas putida.

This section is Part 6 of SN/T 4624.

This section was drafted in accordance with the rules given in GB/T 1.1-2009.

This part is proposed and managed by the National Certification and Accreditation Regulatory Commission.

1 Scope

This part of SN/T 4624 stipulates the morphological identification and hygiene of Staphylococcus aureus for hygiene inspection of microorganisms used for entry into the environment.

Identification, general PCR, and real-time fluorescence PCR detection methods.

This section applies to the inspection and identification of Staphylococcus aureus for the environmental inspection of microbial agents.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document.

GB/T 6682 Analysis Laboratory Water Specifications and Test Methods

3 Main reagents and culture media

3.1 The test water should meet the specifications of the first-grade water in GB/T 6682.

3.2 Normal saline. See Appendix A, A.1.

3.3 7.5% sodium chloride broth. See A.2.

3.4 Blood Agar Plate. See A.3.

3.5 Baird-Parker agar plates. see A.4.

3.6 Brain Heart Leach Broth (BHI). See A.5.

3.7 Rabbit plasma. See A.6.

3.8 Gram staining solution. See A.7.

3.9 Nutrient Agar Slope. See A.8.

3.10 DNA molecular weight markers. 100 bp DNA ladder.

3.11 dNTP. dATP, dTTP, dGTP, dCTP.

3.12 TBE. See A.9.

3.13 TaqDNA polymerase.

3.14 Bacterial genomic DNA extraction kit.

3.15 Agarose.

3.16 Ethidium bromide.

4 Major Instruments and Equipment

4.1 Electronic balance (sensibility 0.01g).

4.2 Incubator. 36 °C ± 1 °C.

4.3 Constant temperature water bath.

4.4 Bench-top refrigerated centrifuge (maximum speed 15000r/min).