SN/T 4624.3-2016Methods for examination of import microbial blends in the environmental protection--Part 3: Bacillus megaterium (English PDF)

Foreword

SN/T 4624 "Method for the detection of microbial agents for environmental protection in entry" is divided into 17 parts.

--- Part 1. Bacillus licheniformis;

--- Part 2. Bacillus pumilus;

--- Part 3. Bacillus megaterium;

--- Part 4. Thiobacillus ferrooxidans;

---Part 5. beta-type hemolytic streptococcus;

--- Part 6. Staphylococcus aureus;

--- Part 7. Salmonella;

--- Part 8. Shigella;

--- Part 9. Escherichia coli;

--- Part 10. Paecilomyces lilacinus;

--- Part 11. elegant small gram silver mold;

--- Part 12. Trichoderma harzian;

--- Part 13. Phanerochaete chrysosporium;

--- Part 14. Aspergillus oryzae;

---Part 15. Bacillus amyloliquefaciens;

--- Part 16. Pseudomonas aeruginosa;

--- Part 17. Pseudomonas putida.

This part is the third part of SN/T 4624.

This part is drafted in accordance with the rules given in GB/T 1.1-2009.

This part is proposed and managed by the National Certification and Accreditation Administration.

This section drafted by. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.

Drafters of this section. Wang Fang, Liu Jiao, Xu Yihong, Jia Lin, He Rui.

Method for detecting microbial agents for environmental protection Part 3. Bacillus megaterium

1 Scope

This part of SN/T 4624 stipulates the morphological identification and biochemistry of Bacillus megaterium for the compliance of microbial agents for environmental protection.

Identification, molecular biology testing methods.

This section applies to the detection and identification of Bacillus megaterium for the compliance of microbial agents for environmental protection.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.

Pieces. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

3 Main reagents and medium

3.1 Experimental water (should meet the specifications of the first grade water in GB/T 6682).

3.2 Nutritional agar. See Appendix A, A.1.

3.3 Nutritional Broth. See A.2.

3.4 Gram staining related reagents. see A.3.

3.5 Contact enzyme. see A.4.

3.6 Oxidase. See A.5.

3.7 Sugar Fermentation Tube. See A.6.

3.8 Citrate. See A.7.

3.9 Nitrate reduction. see A.8.

3.10 Starch hydrolysis. see A.9.

3.11 Gelatin. See A.10.

3.12 TE buffer (pH 8.0). see A.11.

3.13 1%~3% agarose gel. see A.12.

3.14 25mmol/LEDTA. See A.13.

3.15 3 mol/L sodium acetate. see A.14.

3.16 dNTP. dATP, dTTP, dGTP, dCTP.

3.17 TBE. See A.15.

3.18 Taq DNA polymerase.

3.19 Bacterial Genomic DNA Extraction Kit.

3.20 agarose.

3.21 Ethidium bromide.

3.22 DNA molecular weight marker. 100 bp DNA ladder.