SN/T 4624.17-2016Methods for examination of import microbial blends in the environmental protection—Part 17: Pseudomonas putida (English PDF)

Foreword

SN/T 4624 "inbound environmental testing methods using microbial agents" is divided into 17 parts.

--- Part 1. Bacillus licheniformis;

--- Part 2. Bacillus pumilus;

--- Part 3. Bacillus megaterium;

--- Part 4. Acidithiobacillus ferrooxidans;

--- Part 5. beta-hemolytic streptococcus;

--- Part 6. Staphylococcus aureus;

--- Part 7. Salmonella;

--- Part 8. Shigella;

--- Part 9. Diarrhea caused by Escherichia coli;

--- Part 10. Paecilomyces lilacinus;

--- Part 11. Elegant small ginkgo mildew;

--- Part 12. Trichoderma harzianum;

--- Part 13. Phanerochaete chrysosporium;

--- Part 14. Curvularia;

--- Part 15. Bacillus amyloliquefaciens;

--- Part 16. Alkaloid-like-producing Pseudomonas;

--- Part 17. Pseudomonas putida.

This section is part 17 of SN/T 4624.

This section drafted in accordance with GB/T 1.1-2009 given rules.

This part of the national certification and accreditation supervision and management committee proposed and centralized.

This part of the drafting unit. People's Republic of China Liaoning Exit Inspection and Quarantine.

1 Scope

SN/T 4624 provisions of this part of the entry of environmental Microbacteria compatibility test Pseudomonas putida morphological identification, biochemical Identification, ordinary PCR detection method.

This section applies to inbound environmental Microbial agents Pseudomonas putida detection and identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 analytical laboratory water specifications and test methods

3 Main reagents and medium

3.1 Experimental water should be consistent with a water GB/T 6682 specifications.

3.2 Nutrient agar. See Appendix A, A.1.

3.3 Nutritious broth. See A.2.

3.4 Gram staining solution See A.3.

3.5 Starch hydrolysis medium. See A.4.

3.6 chromogenic medium. See A.5.

3.7 TE buffer (pH 8.0). See A.6.

3.8 10 × PCR buffer,.200 mmol/L Tris-HCl (pH 8.4),.200 mmol/L KCl, 15 mmol/L chlorination Magnesium (MgCl2).

3.9 dNTP.dATP, dTTP, dGTP, dCTP.

3.10 TBE.54gTris, 27.5g boric acid, 800mL deionized water dissolved, 20mL0.5mol/LEDTA (pH8.0) fixed volume to 1L.

3.11 Taq DNA polymerase.

3.12 bacterial genomic DNA extraction kit.

3.13 50 × TAE buffer (pH 8.5). See A.7.

3.14 agarose.

3.15 ethidium bromide.

3.16 DNA molecular weight marker .100bp DNAladder.

4 Main equipment and equipment

4.1 electronic balance (sense of volume 0.01g).