SN/T 4624.14-2016Methods for examination of import microbial blends in the environmental protection--Part 14: Aspergillus ustus (English PDF)

Foreword

SN/T 4624 "inbound environmental testing methods using microbial agents" is divided into 17 parts.

--- Part 1. Bacillus licheniformis;

--- Part 2. Bacillus pumilus;

--- Part 3. Bacillus megaterium;

--- Part 4. Acidithiobacillus ferrooxidans;

--- Part 5. beta-hemolytic streptococcus;

--- Part 6. Staphylococcus aureus;

--- Part 7. Salmonella;

--- Part 8. Shigella licheniformis;

--- Part 9. Diarrhea caused by Escherichia coli;

--- Part 10. Paecilomyces lilacinus;

--- Part 11. Elegant small ginkgo mildew;

--- Part 12. Trichoderma harzianum;

--- Part 13. Phanerochaete chrysosporium;

--- Part 14. Curvularia;

--- Part 15. Bacillus amyloliquefaciens;

--- Part 16. Alkaloid-like-producing Pseudomonas;

--- Part 17. Pseudomonas putida.

This section is part 14 of SN/T 4624.

This section drafted in accordance with GB/T 1.1-2009 given rules.

This part of the national certification and accreditation supervision and management committee proposed and centralized.

This part of the drafting unit. Liaoning Entry-Exit Inspection and Quarantine of People's Republic of China, Exit Inspection and Quarantine of Hebei.

1 Scope

SN/T 4624 provisions of this part of the entry of environmental Microbiological Microbiological Compliance test Aspergillus morphology identification, real-time fluorescence PCR detection method.

This section applies to the entry of environmentally friendly microbial inoculum test for the detection and identification of Aspergillus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 analytical laboratory water specifications and test methods

3 Main reagents and medium

3.1 Experimental water should be consistent with a water GB/T 6682 specifications.

3.2 Charles's agar See Appendix A, A.1.

3.3 Char's broth. See A.2.

3.4 CTAB buffer. See A.3.

3.5 Protease K.

3.6 Tris saturated phenol.

3.7 trichloromethane.

3.8 Isoamyl alcohol.

3.9 isopropanol.

3.10 TE buffer (pH 8.0). See A.4.

3.11 70% ethanol.

3.12 ethidium bromide.

3.13 DNA molecular weight marker .1000bp DNAladder.

3.14 agarose.

3.15 50 × TAE buffer (pH 8.5). See A.5.

3.16 RealMasterMix (2.5 ×, ROX containing reference dye).

3.17 20 × ProbeEnhancerSolution.

3.18 liquid nitrogen.

4 Major equipment and materials

4.1 electronic balance (sense of volume 0.01g).