SN/T 4624.12-2016Methods for examination of import microbial blends in the environmental protection--Part 12: Trichoderma harzianum (English PDF)

Foreword

SN/T 4624 "Environmental Use of Microbial Agents for Environmental Protection" is divided into 17 parts.

--- Part 1. Bacillus licheniformis;

--- Part 2. Bacillus pumilus;

--- Part 3. Bacillus megaterium;

--- Part 4. Thiobacillus ferrooxidans;

--- Part 5. Beta hemolytic streptococci;

--- Part 6. Staphylococcus aureus;

--- Part 7. Salmonella;

--- Part 8. Shigella;

--- Part 9. Escherichia coli;

--- Part 10. Paecilomyces lilacinus;

--- Part 11. Elegant Chickwort;

--- Part 12. Trichoderma harzianum

--- Part 13. Phanerochaete chrysosporium;

--- Part 14. Aspergillus coli;

--- Part 15. Bacillus amyloliquefaciens;

--- Part 16. Pseudomonas alkaloids;

--- Part 17. Pseudomonas putida.

This section is Part 12 of SN/T 4624.

This section was drafted in accordance with the rules given in GB/T 1.1-2009.

This part is proposed and managed by the National Certification and Accreditation Regulatory Commission.

1 Scope

This part of SN/T 4624 stipulates the morphological identification and real-time fluorescence of Trichoderma harzianum for the purpose of confirming the conformity of environmental microorganisms used for entry into the country.

PCR detection method.

This section applies to the entry of environmentally friendly microbial agents for the detection of Harmful Hartz test and identification.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article in the case of undated references, the latest version (including all amendments) applies to this document.

GB/T 6682 Analysis Laboratory Water Specifications and Test Methods

3 Main reagents and culture media

3.1 The test water should meet the specifications of the first-grade water in GB/T 6682.

3.2 Potato-dextrose agar (PDA). See Appendix A, A.1.

3.3 Potato-dextrose broth. See A.2.

3.4 CTAB buffer. see A.3.

3.5 Proteinase K.

3.6 Tris saturated phenol.

3.7 Chloroform.

3.8 Isopentanol.

3.9 Isopropyl alcohol.

3.10 TE buffer (pH 8.0). See A.4.

3.11 70% ethanol.

3.12 Ethidium bromide.

3.13 DNA molecular weight markers. 1000 bp DNA ladder.

3.14 Agarose.

3.15 50 x TAE buffer (pH 8.5). see A.5.

3.16 RealMasterMix (2.5x with ROX internal reference dye).

3.17 20×ProbeEnhancerSolution.

3.18 liquid nitrogen.

4 Major equipment and materials

4.1 Electronic balance (sensibility 0.01g).

4.2 Incubator. 25 °C ± 1 °C.