SN/T 3767.2-2014Loop-mediated isothermal amplification detection method for genetically modified components in food for export - Part 2: Screening method (English PDF)

Foreword

SN/T 3767 "genetically modified food for export loop-mediated isothermal amplification (LAMP) detection method" is divided into 30 parts.

Part 1. General requirements and definitions;

Part 2. The screening method;

Part 3. maize Bt-11 strain;

Part 4. Bt176 maize lines;

Part 5. GA21 maize lines;

Part 6. MIR162 Maize Lines;

Part 7. MIR604 maize lines;

Part 8. maize MON810 strain;

Part 9. maize MON863 strain;

Part 10. maize MON88017 strain;

Part 11. maize MON89034 strain;

Part 12. Corn T-25 strain;

Part 13. 3272 maize lines;

Part 14. Lines of corn 59122;

Part 15. A2704-12 soybean lines;

Part 16. Soybean A5547-127 lines;

Part 17. Soybean DP356043 lines;

Part 18. Soybean GTS40-3-2 lines;

Part 19. Soybean MON89788 strain;

Part 20. Rice Bt-63 strain;

Part 21. Rice KF6 lines;

Part 22. Rice KF8 lines;

Part 23. KMD rice strains;

Part 24. Rice LLRICE62 lines;

Part 25. Rice M12 strain;

Part 26. Rice T1C-19 strain;

Part 27. Rice T2A-1 strain;

Part 28. B73-6-1 wheat strain;

Part 29. beet H7-1 strain;

Part 30. Rape RT-73 strain.

This section SN/T Section 23767 of.

This section drafted in accordance with GB/T 1.1-2009 given rules.

Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.

This section proposed and managed by the National Certification and Accreditation Administration Committee.

1 Scope

SN/T 3767 provisions of this part of the plant-derived foods genetically modified loop-mediated isothermal amplification (LAMP) screening test method.

This section applies to the import and export of rice, qualitative detection of maize, soybeans, potatoes, wheat and its processing products.

Qualitative detection limit of this method was 0.5% (mass fraction).

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.2 technical requirements for GMO testing laboratories GB/T 19495.3 GMO detection of nucleic acid extraction and purification method GB/T 19495.7 GMO detection methods of sampling and sample preparation SN/T 1204 GMO plants and their processed products in real-time PCR qualitative test methods

3 Abbreviations

The following abbreviations apply to this document.

PLD. phospholipaseDgene, phospholipase D gene ZEIN. zein gene Lectin. gene ST-LS1. potato light-inducible tissue-specific gene GAG56D. the case of wheat gliadin gene CaMV35S. 35Spromoterfromcauliflowermosaicvirus, derived from the cauliflower mosaic virus 35S promoter NOS. terminatorofnopalinesynthasegene, nopaline synthase gene terminator FMV35S. 35Spromoterfromamodifiedfigwortmosaicvirus (caulimovirusgroup), derived from the flower Scrophulariaceae Mosaic virus 35S promoter NPTII. neomycin-3'-phosphotransferasegene, 3'neomycin, phosphotransferase gene BAR. phosphinothricinacetyltransferasegenefromBacillusamyloliquefaciens, derived from Bacillus Ba-cillusamyloliquefaciens glufosinate acetyltransferase gene PAT. phosphinothricinacetyltransferasegene, glufosinate acetyltransferase gene GOX. glyphosateoxidoreductasegene, glyphosate oxidoreductase gene LAMP. loop-mediatedisothermalamplification, loop-mediated isothermal amplification