SN/T 3691-2013PCR-DHPLC method for detection of genetically modified corn (English PDF)
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Issued by
General Administration of Customs
Level / Type
National · Recommended
Issue date
August 30, 2013
Implementation date
March 1, 2014
Scope
SN/T 3691-2013 (PCR-DHPLC method for detection of genetically modified corn) is available as an English-translated PDF.
SN/T 3691-2013 - This standard specifies the PCR-DHPLC detection of transgenic maize product ingredients. This standard applies to products transgenic corn line 59122, Bt11, Bt176, CBH351, EV3272, GA21, MIR604, MON810, MON863, MON88017, MON89034, NK603, T25 and TC1507 detection.
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Document preview — SN/T 3691-2013
National Standard of the People's Republic of China
Issued by: General Administration of Customs
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms, definitions and abbreviations
- 3.1 Terms and Definitions
- 3.2 Acronyms
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, China Inspection Quarantine Science Research Institute, National Center for Nanoscience, Shenzhen Bo Rui Xiang Hui Biotechnology Co., Ltd., Liaoning Academy of Agricultural Sciences.
1 Scope
This standard specifies the PCR-DHPLC detection of transgenic maize product ingredients.
This standard applies to products transgenic corn line 59122, Bt11, Bt176, CBH351, EV3272, GA21, MIR604, MON810, MON863, MON88017, MON89034, NK603, T25 and TC1507 detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.2 technical requirements for GMO testing laboratories GB/T 19495.7 GMO detection methods of sampling and sample preparation SN/T 1193 genetic testing laboratory technical requirements
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
The following terms and definitions apply to this document.
Denaturing high-performance liquid chromatography (DenaturingHigh-performanceLiquidChromatography, DHPLC) an application of the principle of ion-pair reversed-phase liquid chromatography separation of DNA fragments is simple, fast, non-gel method for nucleic acid analysis.
3.2 Acronyms
The following abbreviations apply to this document.
BAR. phosphate tricine acetyltransferase gene (phosphinothricinacetyltransferasegene) bp. base pairs (basepair) CaMV35S. cauliflower mosaic virus 35S promoter (35Spromoterfromcauliflowermosaicvirus) Ct value. number of cycles in each reaction tube fluorescent signal reaches the set threshold experienced (C. Cycle, t. threshold) CTAB. cetyl trimethyl ammonium bromide (cetyltrithylammoniumbromide) dATP. triphosphate (deoxyadenosinetriphosphate) dCTP. deoxycytidine triphosphate (deoxycytidinetriphosphate) dGTP. deoxyguanosine triphosphate (deoxyguanosinetriphosphate) DNA. deoxyribonucleic acid (deoxyribonucleicacid) dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate) dTTP. deoxythymidine triphosphate (deoxythymidinetriphosphate) dUTP. deoxyuridine triphosphate (deoxyuridinetriphosphate) EDTA. ethylenediaminetetraacetic acid (ethylenediaminetetraaceticacid) FMV35S. figwort mosaic virus 35S promoter (35SpromoterfromFigwortMosaicVirus)
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 7 pages — is available in the English PDF.
Referenced standards
Normative references
- GB/T 6682Water for analytical laboratory use - Specification and test methods
- GB/T 19495.2Detection of genetically modified organisms and derived products--General requirements for laboratories
- GB/T 19495.7Detection of genetically modified organisms and derived products--Methods for sampling and sample preparation
SN/T 1193
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Related Standards
GB/T 19495.2-2004 — Detection of genetically modified organisms and derived products--General requirements for laboratories
GB/T 19495.7-2004 — Detection of genetically modified organisms and derived products--Methods for sampling and sample preparation
GB/T 6682-2008 — Water for analytical laboratory use - Specification and test methods
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SN/T 3691-2013
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