SN/T 3330-2012Protocol of identification for Tachypleus tridentatus. PCR method (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

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This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Yantai Exit Inspection and Quarantine, People's Republic of China Shandong Entry-Exit Inspection and Quarantine Bureau.

1 Scope

This standard specifies the Chinese horseshoe crab species by PCR method.

This standard applies to entry and exit aquatic and biological preparations containing Chinese horseshoe crab ingredient identification of Chinese horseshoe crab.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

Laboratory use specifications and test methods GB/T 6682 Analysis SN/T 1193 genetic testing laboratory technical requirements

3 Terms and Definitions

The following terms and definitions apply to this document.

Polymerase chain reaction polymernasechainreaction; PCR in thermostable DNA polymerase and a pair of primers (tested with target nucleic acid molecule sequences homologous DNA fragments) at high temperature (DNA points Sub-denatured) and low temperature (primer profit target nucleic acid molecule complex nature and thermostable DNA polymerase extension) alternating cycles of amplification of the target nucleic acid test Child approach.

Principle 4

Chinese horseshoe crab (morphological characteristics see Appendix A) sample after extraction of total genomic DNA, the use of specific primers designed by conventional PCR specific amplification technology Chinese horseshoe crab mtDNACOX3 gene fragment (349bp). Chinese horseshoe crab samples is determined according to the results of PCR amplification is Chinese horseshoe crab species. Specific primers by mtDNA than Chinese horseshoe crab, Limulus round tail, American horseshoe crab, Limulus and other mostly southern horseshoe crab COX3 gene fragments designed.

5 Equipment and Reagents