SN/T 3196-2012Determination of pathogenic vibrio in aquatic product. Automatic detection system for screening pathogenic bacteria (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

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This standard is proposed and managed by the National Certification and Accreditation Administration Committee.

This standard was drafted. People's Republic of China Fujian Entry-Exit Inspection and Quarantine Zhanjiang, People's Republic of China Bureau, People's Republic of China Shanghai Entry-Exit Inspection and Quarantine, Ningbo People's Republic of China.

1 Scope

This standard specifies the aquatic Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus screening methods.

This standard applies to Vibrio cholerae in aquatic products, rapid screening detection of Vibrio parahaemolyticus and Vibrio vulnificus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

GB/T 4789.7 Microbiological examination of food hygiene Vibrio parahaemolyticus test Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 27403 laboratory quality control of food molecular biology standardized testing SN/T 0173 Importers and Vibrio parahaemolyticus in food testing methods SN/T 1022 Vibrio cholerae in food import and export inspection methods FDA/BAMChapter9 FDA Microbiological Analysis Handbook Chapter 9 Vibrio NMKLNo.156 Nordic Food Association food pathogenic Vibrio qualitative and quantitative detection methods

3 Abbreviations

The following abbreviations apply to this document.

5 Reagents

5.1 experimental water should comply with GB/T 6682 in a water specifications.

5.2 Alkaline peptone water (APW), see A.1.

5.3 BAX system of Vibrio cholerae/parahaemolyticus/vulnificus the PCR detection kit, 2 °C ~ 8 °C place, see A.2.