SN/T 2497.27-2010Test method of import and export dangerous chemicals. Part 27: The usage of flow cytometry in apoptosis (English PDF)

Foreword

SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.

--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;

--- Part 2. plaque-forming cells (PFC) test;

--- Part 3. Large Zhuan reproduction test;

--- Part 4. Saccharomyces cerevisiae mitotic recombination test;

--- Part 5. testicular cells UDS test;

--- Part 6. mammalian cells in vitro sister chromatid exchange test;

--- Part 7. mouse ear swelling test;

Part --- Article 8. fossa lymph node assay;

--- Part 9. Determination of serum hemolysin test;

Part --- Article 10. T lymphocyte proliferation assay test;

--- Part 11. germline mutation assay;

--- Part 12. single cell gel electrophoresis test;

--- Part 13. fluorescence in situ hybridization;

Part --- Article 14. SDS- polyacrylamide gel electrophoresis;

--- Part 15. PCR-SSCP experiment;

Part --- Article 16. Western-Blot test;

--- Part 17. Behavior of mammalian toxicology tests;

--- Part 18. Detection of DNA adducts;

--- Part 19. NorthernBlot experiment;

--- Part 20. Bradford protein assay;

--- Part 21. agarose gel electrophoresis;

--- Part 22. Tm value determination method of DNA;

--- Part 23. organelle separation experimental methods;

--- Part 24. cellular immunity in vitro assay method;

--- Part 25. humoral immune function tests;

--- Part 26. macrophage function tests;

--- Part 27. Flow cytometry of apoptosis;

--- Part 28. Application shuttle plasmid mutations Inspection;

--- Part 29. Biochemical Oxygen Demand (BOD) was measured.

This part is Part 27 SN/T 2497 series of standards.

The partial modification of the use of "Toxicology test methods and technology" in the flow cytometry method of technical content and the method described above End Full agreement on a standard text format according to GB/T 1.1 made editorial changes.

Appendix A of this section is an informative annex.

This section proposed and managed by the National Certification and Accreditation Administration Committee.

This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China Quarantine Bureau.

1 Scope

SN/T 2497 This section specifies the terms and definitions import and export of hazardous chemicals flow cytometry apoptosis, test methods and conclusions Analysis of fruit.

This section applies to the import and export of hazardous chemicals flow cytometry apoptosis test.

2 Terms and definitions

The following terms and definitions apply to this section.

Also known as "programmed cell death", a phenomenon of natural causes cells to environmental stimuli, gene regulation under produced. withered Death was originally determined by characteristic morphological changes, including. procedural degradation of chromatin DNA concentration, cells shrink and crack. it can Physiological or induced by the chemotherapy and radiation.

Vivo local tissue, cell death called necrosis. Metabolism necrotic tissue cells stop, loss of function. Morphological changes of necrosis may So the damage caused by the degradation of intracellular hydrolase, the hydrolysis can also be caused by the migration of white blood cells to release enzymes.

Liquid is suspended cells or particles, physical properties and chemical properties, such as cell size, internal structure with flow cytometry measurements, DNA, RNA, proteins, antigens for rapid determination of a kind of modern analytical techniques can be categorized collection.

Test Method 3