SN/T 2497.22-2010Test method of import and export dangerous chemicals. Part 22: Determination of DNA Tm (English PDF)

Foreword

SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.

--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;

--- Part 2. plaque-forming cells (PFC) test;

--- Part 3. Large Zhuan reproduction test;

--- Part 4. Saccharomyces cerevisiae mitotic recombination test;

--- Part 5. testicular cells UDS test;

--- Part 6. mammalian cells in vitro sister chromatid exchange test;

--- Part 7. mouse ear swelling test;

--- Part 8. popliteal lymph node assay;

--- Part 9. Determination of serum hemolysin test;

Part --- Article 10. T lymphocyte proliferation assay test;

--- Part 11. germline mutation assay;

--- Part 12. single cell gel electrophoresis test;

--- Part 13. fluorescence in situ hybridization;

Part --- Article 14. SDS- polyacrylamide gel electrophoresis;

--- Part 15. PCR-SSCP experiment;

Part --- Article 16. Western-Blot test;

--- Part 17. Behavior of mammalian toxicology tests;

--- Part 18. Detection of DNA adducts;

--- Part 19. NorthernBlot experiment;

--- Part 20. Bradford protein assay;

Part --- Zi 21. agarose gel electrophoresis;

--- Part 22. Tm value determination method of DNA;

--- Part 23. organelle separation experimental methods;

--- Part 24. cellular immunity in vitro assay method;

--- Part 25. humoral immune function tests;

--- Part 26. macrophage function tests;

--- Part 27. Flow cytometry of apoptosis;

--- Part 28. Application shuttle plasmid mutations Inspection;

--- Part 29. Biochemical Oxygen Demand (BOD) was measured.

This part is Part 22 SN/T 2497 series of standards.

This section proposed and managed by the National Certification and Accreditation Administration Committee.

This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China Quarantine Bureau.

1 Scope

SN/T 2497 provisions of this part of the test sample and export of dangerous chemicals in the test Test Tm value of DNA, Solution, test procedures and test results.

This section applies to the import and export of dangerous chemicals Tm value measurement test DNA.

Principle 2

DNA in when the temperature rose to a certain value, the hydrogen bonds between the two single-stranded nucleotide began to be opened (DNA begin to denature) Separation, so that the DNA solution is significantly increased absorption in the ultraviolet at a wavelength of 260nm; When the temperature up to a certain value, complete separation of DNA Into single strands, then continue to heat, UV absorption of DNA solution is no longer increases. When DNA thermal denaturation UV absorption maximum increase Thermal denaturation temperature or melting temperature of the temperature corresponding to half of the DNA is called, i.e. the Tm value.

3 Reagents

4 Test Procedure