SN/T 2497.18-2010Test method of import and export dangerous chemicals. Part 18: Detection method of DNA adduct (English PDF)

Foreword

SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.

--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;

--- Part 2. plaque-forming cells (PFC) test;

--- Part 3. Large Zhuan reproduction test;

--- Part 4. Saccharomyces cerevisiae mitotic recombination test;

--- Part 5. testicular cells UDS test;

--- Part 6. mammalian cells in vitro sister chromatid exchange test;

--- Part 7. mouse ear swelling test;

Part --- Article 8. fossa lymph node assay;

--- Part 9. Determination of serum hemolysin test;

Part --- Article 10. T lymphocyte proliferation assay test;

--- Part 11. germline mutation assay;

--- Part 12. single cell gel electrophoresis test;

--- Part 13. fluorescence in situ hybridization;

Part --- Article 14. SDS- polyacrylamide gel electrophoresis;

--- Part 15. PCR-SSCP experiment;

Part --- Article 16. Western-Blot test;

--- Part 17. Behavior of mammalian toxicology tests;

--- Part 18. Detection of DNA adducts;

--- Part 19. NorthernBlot experiment;

--- Part 20. Bradford protein assay;

--- Part 21. agarose gel electrophoresis;

--- Part 22. Tm value determination method of DNA;

--- Part 23. organelle separation experimental methods;

--- Part 24. cellular immunity in vitro assay method;

--- Part 25. humoral immune function tests;

--- Part 26. macrophage function tests;

--- Part 27. Flow cytometry of apoptosis;

--- Part 28. Application shuttle plasmid mutations Inspection;

--- Part 29. Biochemical Oxygen Demand (BOD) was measured.

This is Part 18 SN/T 2497 series of standards.

This section proposed and managed by the National Certification and Accreditation Administration Committee.

This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China Quarantine Bureau.

1 Scope

SN/T 2497 provisions of this part of the main reagents and materials import and export of hazardous chemicals DNA adduct detection methods, the main instrument Device, test procedures and test results.

This section applies to post-32P labeled DNA adducts were detected due to the import and export of hazardous chemicals.

2 Terms, definitions and abbreviations

4 Reagents and materials

Unless otherwise specified, all reagents were of analytical grade, sterile distilled water was used or ultrapure water.

Endonuclease 4.1.

4.2 exonuclease.

4.3 P1 nuclease.

4.4 32P-ATP.

4.5 T4 polynucleotide kinase.

4.6 T4 polynucleotide Kinase Buffer. 0.1mol/LBicine-NaOH, 0.1mol/LMgCl2,0.1mol/L-threitol disulfide, 10mmol/L spermidine, pH value of 9.5.

4.7 ODS-TLC plate (reverse phase adsorption TLC plate).

4.8 PEI-TLC plate (TLC plate anion exchange).

5 Key Equipment

5.1 UV spectrophotometer.