SN/T 2497.15-2010Test method of import and export dangerous chemicals. Part 15: Test of PCR-SSCP (English PDF)

Foreword

SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.

--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;

--- Part 2. plaque-forming cells (PFC) test;

--- Part 3. Large Zhuan reproduction test;

--- Part 4. Saccharomyces cerevisiae mitotic recombination test;

--- Part 5. testicular cells UDS test;

--- Part 6. mammalian cells in vitro sister chromatid exchange test;

--- Part 7. mouse ear swelling test;

Part --- Article 8. fossa lymph node assay;

--- Part 9. Determination of serum hemolysin test;

Part --- Article 10. T lymphocyte proliferation assay test;

--- Part 11. germline mutation assay;

--- Part 12. single cell gel electrophoresis test;

--- Part 13. fluorescence in situ hybridization;

Part --- Article 14. SDS- polyacrylamide gel electrophoresis;

--- Part 15. PCR-SSCP experiment;

Part --- Article 16. Western-Blot test;

--- Part 17. Behavior of mammalian toxicology tests;

--- Part 18. Detection of DNA adducts;

--- Part 19. NorthernBlot experiment;

--- Part 20. Bradford protein assay;

--- Part 21. agarose gel electrophoresis;

--- Part 22. Tm value determination method of DNA;

--- Part 23. organelle separation experimental methods;

--- Part 24. cellular immunity in vitro assay method;

--- Part 25. humoral immune function tests;

--- Part 26. macrophage function tests;

--- Part 27. Flow cytometry of apoptosis;

--- Part 28. Application shuttle plasmid mutations Inspection;

--- Part 29. Biochemical Oxygen Demand (BOD) was measured.

This is Part 15 SN/T 2497 series of standards.

This section proposed and managed by the National Certification and Accreditation Administration Committee.

This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China Quarantine Bureau.

1 Scope

SN/T 2497 provisions of this part of the main reagents and materials import and export of hazardous chemicals PCR-SSCP experiments, the main instruments provided Equipment, test procedures and test results.

This section applies to screening DNA fragments can cause mutation of the import and export of hazardous chemicals.

2 Terms, definitions and abbreviations

4 Reagents and materials

Unless otherwise specified, all reagents were of analytical grade, sterile distilled water was used or ultrapure water.

4.1 30% Acrylamide. Acrylamide 29g, N, N'- methylene bisacrylamide 1g, add distilled water to 60mL, heated to 37 [deg.] C to dissolve, Additional water to a final volume of 100mL, filter sterilized.

4.2 5 × TBE. 54gTris base, 27.5g boric acid, 20mL0.5mmol/LEDTA, adjusting the pH to 8.0.

4.3 10% ammonium persulfate. 1g ammonium persulfate was dissolved in 10mL of water, 4 °C stored for several weeks.

4.4 TEMED.

4.5 6 × sample loading buffer. 0.25% bromophenol blue, 0.25% xylene green FF, 30% glycerol solution, 4 °C preservation.