SN/T 2206.11-2014Determination of microorganism in cosmetics. Part 11: Multiplex fluorescent real-time PCR method for Staphylococcus aureus (English PDF)
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Issued by
General Administration of Customs
Level / Type
National · Recommended
Issue date
April 9, 2014
Implementation date
November 1, 2014
Scope
SN/T 2206.11-2014 (Determination of microorganism in cosmetics. Part 11: Multiplex fluorescent real-time PCR method for Staphylococcus aureus) is available as an English-translated PDF.
SN/T 2206.11-2014 — This section SN/T 2206 specifies the multiplex real-time PCR for detection of Staphylococcus aureus in cosmetics. This section applies to cosmetics qualitative detection of Staphylococcus aureus.
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Document preview — SN/T 2206.11-2014
National Standard of the People's Republic of China
- ICS
- 71.100.70
- Classification
- Y 42
Issued by: General Administration of Customs
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms, definitions and abbreviations
- 3.1 Terms and Definitions
- 3.2 Acronyms
- 4 Detection
- 4.1 Principle of the method
Foreword
SN/T 2206 "cosmetic microbiological test method" is divided into 13 parts.
--- Part 1. Salmonella;
--- Part 2. aerobic Bacillus subtilis and Bacillus cereus;
--- Part 3. Klebsiella pneumoniae;
--- Part 4. Streptococcus;
--- Part 5. Enterococcus;
--- Part 6. Clostridium tetani;
--- Part 7. Western blot detection of BSE pathogen;
--- Part 8. Candida albicans;
--- Part 9. bile acid tolerance Gram-negative bacteria;
--- Part 10. Staphylococcus aureus PCR method;
--- Part 11. Staphylococcus aureus multiplex real-time PCR method;
--- Part 12. Pseudomonas aeruginosa PCR method;
--- Part 13. maltophilia Aeromonas.
This section SN/T 2206 Part 11.
This section drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Posted structure of this document does not assume responsibility for the identification of these patents.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This part mainly drafted by. People's Republic of China Shandong CIQ.
The main part of the main drafters. Xu Hongyan, Liu Ning, Wang Ying, Yin Wei force, Lu Min, Duan Hui efficiency, Fangshao Qing, Cao Peng, Gengjin Pei.
Cosmetic microbiological test method Part 11. Staphylococcus aureus Multiplex real-time PCR method
1 Scope
This section SN/T 2206 specifies the multiplex real-time PCR for detection of Staphylococcus aureus in cosmetics.
This section applies to cosmetics qualitative detection of Staphylococcus aureus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682-2008 Analysis GB 7918.1 General Cosmetics Standard methods of microbiological GB 7918.5 Microbiological Criteria for cosmetic testing methods Staphylococcus aureus SN/T 1193 gene laboratory technical requirements
3 Terms, definitions and abbreviations
The following terms, definitions and abbreviations apply to this document.
3.1 Terms and Definitions
3.1.1
Ct values CyclethresholdCt when the number of cycles the fluorescence signal of each reaction tube reaches the set threshold experienced.
3.2 Acronyms
Taq. Thermus aquaticus bacteria (Thermusaquaticu) FAM. 6- carboxyfluorescein (6-carboxyfluorescein) HEX. 6- carboxy-2 ', 4,4', 5 ', 7,7'-hexachlorofluorescein (5-hexachloro-fluorescein) BHQ. black hole quencher (Blackholequencher)
4 Detection
4.1 Principle of the method
Join pairs of primers and the template DNA matching both ends oligonucleotide probe fluorophore-labeled PCR reaction. PCR the number of new chains once every cycle, and the number of released synthetic fluorophores were correspondence relationship, i.e., the amount of PCR product with the intensity of the fluorescence signal Was correspondence. When the fluorescence signal exceeds the set threshold, the fluorescence signal can be detected, increase the amount of the instrument can detect fluorescence signals
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 6 pages — is available in the English PDF.
Referenced standards
Normative references
GB 7918.1 · GB 7918.5 · SN/T 1193
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Related Standards
GB/T 6682-2008 — Water for analytical laboratory use - Specification and test methods
SN/T 2206.13-2014 — Determination of microorganism in cosmetics - Part 13: Stenotrophomonas maltophilia
SN/T 2206.14-2017 — Determination of microbiologies in cosmetics. Part 14: Staphylococcus saprophyticus
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