SN/T 1960-2007Screening method of sulfonamide residues in animal original food for import and export. Enzyme linked immunosorbent assay method (English PDF)

3.1 Method summary

The samples were extracted with sulfa drugs extracts were detected using a competitive enzyme-linked immunosorbent assay.

3.2 Reagents and materials

Unless otherwise requested, all reagents were of analytical grade water as a water GB/T 6682 regulations.

3.2.1 sulfa drugs ELISA assay kit 1) (see Appendix A).

1) sulfa drugs ELISA kit provided by the Tianjin Biochip Technology Co., Ltd. under the trade name product. This gives Information is for the convenience of users of this standard does not imply endorsement of the product. If other equivalent product has the same effect, you can use this Some equivalent.

3.2.2 acetone.

3.2.3 ethanol.

3.2.4 disodium hydrogen phosphate (Na2HPO4 · 12H2O).

3.2.5 sodium dihydrogen phosphate (NaH2PO4 · 2H2O).

3.2.6 sodium chloride.

3.2.7 masking agent dilution. 0.5% bovine serum albumin, 1% bovine serum albumin, when used in the preparation.

3.2.8 Standard. sulfamethazine different mouth oxazole, Sulfathiazole, sulfamethoxydiazine, sulfa methoxy-triazine, sulfasalazine, sulfamethizole, sulfonamides Chlorine of triazine (English names are. sulfisozole, sulfathiazole, sufameter, sulfamethoxypyridazine, sulfapyridine, sulfamethizole, sulfachlorpyridazine; CAS Code. 127-69-5,72-14-0,651-06-9,80-35-3,144-83-2, 144-82-1,80-32-0). not less than 99% purity.

3.2.9 Standard stock solution. formulated as 10mg/L stock solution of methanol, -20 °C preservation, valid for three months.

Cryopreservation agents, stand at room temperature should not exceed 4h, when used, should each solution to reach room temperature.

3.3 Instruments and equipment

3.3.1 grinder.

3.3.2 homogenizer.

3.3.3 scales (accurate to 0.001g).

3.3.4 vortex mixer.