SN/T 1470-2004Examination codes for dengue fever at frontier port (English PDF)

5.1 Specimen collection and processing

5.1.1 Dengue patients, suspected dengue patients and other personnel

Collect 3 mL of venous blood within 5 days after onset, set it to solidify at room temperature, centrifuge for 15 min at 3000 r/min, and aspirate the upper serum for examination.

Measurement. The virus should be separated using the same day serum. For contaminated serum, penicillin and streptomycin should be added first (the final concentration is 500kU/L), and 4°C is used.

Inoculate after 2h treatment. The remaining serum after inoculation was early serum. Aseptic collection of blood samples 3 weeks after the disease, treated as above, as a recovery Reconstituted serum for serological testing.

5.1.2 Suspected dengue deaths

Blood, cerebrospinal fluid, brain tissue, liver tissue, etc. are available. The blood and cerebrospinal fluid were treated as in 5.1.1. Tissue block grinding to make suspension, 3000r/min After centrifugation for 15 min, the supernatant was taken for detection.

5.2 Request for inspection of specimens

5.2.1 The identification of the sample to be inspected shall be consistent with the records of the inspection form and the test list.

5.2.2 The container for the specimen to be inspected shall be free from damage and leakage.

5.2.3 Blood specimens should be free of hemolysis, hyperlipidemia and jaundice.

5.2.4 Remote inspection specimens should be refrigerated.

5.3 Specimen preservation

If the separated serum cannot be detected immediately, it can be stored under the following conditions.

--- Detected within 3d, can be stored at 2 ° C ~ 8 ° C;

--- Short-term storage can be stored below -20 °C;

--- Long-term storage should be kept below -70 ° C;