SN/T 1148-2019Cassava single-claw quarantine identification method (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

This standard replaces the quarantine and identification method of single-clawed mite of cassava by SN/T 1148-2002.

The main content changes are as follows.

--- Added approximate species differences and related search tables;

--- Added relevant information such as distribution location;

--- Added morphological feature map.

This standard is proposed and managed by the General Administration of Customs of the People's Republic of China.

1 Scope

This standard is applicable to the quarantine and identification of single-clawed mites carrying cassava in imported plants and their products.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document.

SN/T 2122 Sampling Methods for Entry and Exit Plants and Plant Products Quarantine

3 Terms and definitions

The following terms and definitions apply to this document.

Horny plate on the back of the body.

Collective term for setae on the back of the body. Bristles born on the back of the limbs.

It is distributed in the body and appendages, it is slender, the tip is thin, the wall is thick, it is slightly fluffy and has tactile function.

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The thick and long part is sensation hair, and the thin and short base hair is tentacle hair.

3.10

The ends of the hairs swell and grow on the crotch claws or interclaw processes. It can secrete mucus and make the mite stick to the surface when walking.

3.11

3.12

A bristle at the end of the fimbriae, thick, cylindrical or conical.

3.13

3.14

3.15

The first row of bristles outside the hind body is usually two. Refers to the shoulder area or setae on the shoulder plate.

3.16

4 Basic Information

See Appendix A for additional information on Monochaete cassava.

5 Method principle

Observe the host leaf at the quarantine site according to the hazards of the single-clawed mite of cassava, obtain the adult mites or obtain them in the laboratory, and dissect them SN/T 1144-201919 ????? ????, ????, ??

The specimens of adult mite slides were observed with a microscope, and the types were determined based on morphological characteristics.

6 Equipment and reagents

8 Laboratory testing

9 Identification characteristics

10 Result judgment

It meets the identification characteristics of 9.3.1, and can be judged to be cassava mite, 9.3.2 and 9.3.3 as reference.

11 Sample Record

The identified specimens of the single-headed mites of Cassava should be kept permanently, and the time, place, host, and collector should be noted.

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Appendix A

(Informative appendix) Relevant information of Monochaete cassava A. 1 Geographical distribution Antigua and Barbuda, Dominican Republic, United States, Mexico, Trinidad and Tobago, Australia, Northern Mariana Islands, Micronesia, Mali, Guinea, Sierra Leone, Liberia, Ghana, Togo, Benin, Niger, Nigeria, Cameroon, Chad, China Africa, Sudan, Ethiopia, Somalia, Kenya, Uganda, Tanzania, Rwanda, Burundi, Zaire, Congo, Gabon, Angola, Zambia, Malawi, Zimbabwe, Côte d'Ivoire, Burkina Faso, South Africa, Argentina, Paraguay, Brazil, Puerto Rico, Bolivia, Ecuador Guador, Guyana, Colombia, Malvinas Islands, Peru, Suriname, Venezuela, Uruguay, Chile and other countries.

Cassava plantations have been found in Hainan, Guangxi, Guangdong, and parts of Yunnan.

A. 2 host plants

Cubicaee, Passifloraceae, Passionaceae, Malvacae of Malvaceae, and Rubiaceae of Rubiaceae.

A. 3 Biology and transmission the cassava single-clawed mite's life history is divided into five stages. egg, juvenile mite, former nymph, posterior nymph and adult mite. Often spawns on the back of the leaf along the midrib to other leaves Veins or leaf depressions, sucking plant buds, buds, new leaves and young stem juice with acupuncture.

In the field, its spreading methods are crawling and spreading with the wind; and its main spreading path is human activity and parasite plant transmission.

A. 4 The hazards of Monochaete cassava A large number of yellow spots appeared on the blocked roots of the plant after the injury, and then expanded into a mosaic pattern, which became deformed. When severely injured, the leaves withered yellow Shedding, green shoots loss, stems appear curved, scars, gradually dry and rough, brown, and finally the plants die and the roots rot.

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Appendix B

(Informative appendix) Reagent preparation method B. 1 Preparation method of Austenitic preservation solution Mix 70 mL of ethanol 87 mL, 8 mL of glacial acetic acid, and 5 mL of glycerol.

B. 2 Preparation method of Nessler's solution Mix 40g of chloral hydrate, 25mL of distilled water, and 2.5mL of concentrated hydrochloric acid.

B. 3 Preparation method of Huo's mounting solution Dissolve 30g of gum arabic in 50mL of distilled water. After it is completely dissolved, put it in a water bath at 40 °C ~ 50 °C and add.200g.

Trichloroacetaldehyde hydrate, stir until dissolved, add 20g of glycerin, and finally filter with a suction funnel or fine cotton cloth.

B. 4 Preparation method of Ai Cai's mounting solution Add 40mL to 60mL of distilled water in 10g of polyvinyl alcohol, heat on a 100 ° C water bath, stir until completely dissolved, and then add 85 to 92% lactic acid 35mL and 10mL glycerin, stir well, cool to 40 °C ~ 50 °C, add 25mL 1.5% phenol aqueous solution and 20 g of trichloroacetaldehyde hydrate, and finally filter with an air extraction funnel or fine cotton cloth.

Note. Unless otherwise specified, all reagents are of analytical grade.

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Appendix C

(Informative appendix) Preparation of slide specimens Use a circular needle to pick the mite specimen from the preservation solution, put it in a Petri dish containing Neisser's solution, and soak it for 24 hours. After soaking mites Pick it up on absorbent paper and use a small brush to dip the Ness solution to clean the specimen. Place a drop of mounting fluid in the center of a clean glass slide.

It is advisable to just cover the coverslip. Use a dissecting needle dipped in the mounting solution to quickly remove the mite specimen from the absorbent paper into the glue solution. Finish the mite Immerse in the bottom layer of the mounting fluid instead of floating on the surface. Posture under a stereo microscope so that the mites have their backs up and their legs extended.

When placed in a mounting medium, the male penis can be fully displayed. Apply a small amount of mounting solution to the center of the coverslip and clamp the cover with tweezers the slide is pressed against the slide in parallel, so that the mounting liquid on the cover glass and the mounting agent on the slide are in parallel contact, and the mounting liquid is evenly spread around. will the prepared slide is observed under a stereo microscope. If the left and right eyes of the front and side male mites are completely overlapped, the position of the male mite penis is right Suitable. Place the slide on the flame of the alcohol lamp and move it to heat. When the mounting fluid on the slide is boiling, remove it immediately to allow the mite body to finish.

Fully stretched and transparent. The slides were placed in an oven at 55 ° C for 2-7 days until they were completely dry. In order to prevent the mounting fluid from getting damp when it is wet, cover the glass with Canadian glue.

The film was sealed around.

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Appendix D

(Informative appendix) Tetranychus mite model and male genus characteristic diagram

Figure C. 2 The ventral view of spider mite (quoted from Economic Entomology)

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Figure C. 3 Jaw body of spider mite (quoted from Economic Entomology)

Figure C. 5 Characteristic map of male genitalia of Monosauria cassava (imitation of J. GUTIERERY, 1978)

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Appendix E

(Informative appendix) Retrieval Form for Common Tetranychus in Cassava E. 1 Retrieval form of common spider mite species that harm cassava plants the hairs of the foot iliac joint are normal;

Terminal body with 1 pair of anal side hairs 3 SN/T 1144-201919 ????? ????, ????, ??

Appendix F

(Informative appendix) Retrieval form of Monochaeta cassava and its approximate species F. 1 Retrieval form of Monochaete cassava and its approximate species the back stripes are not connected; there may be a slight reticulation at the back; foot I tibial has 8 or 9 tentacles and 1 sensory hair 2 2. The hair in the back of the back body is about 1/2 of the length of the hair at the base; the tibial portion of the foot has 9 tentacles and 1 slender hair.

All back hairs are of equal length; foot I tibial has 8 or 9 tentacles and 1 slender hair 3 3. Body hair is long, thick, jagged, and swollen at the ends. The length of the back hair is approximately the same except for the shorter front half body shoulder hair and shoulder hair which is slightly shorter; foot I SN/T 1144-201919 ????? ????, ????, ??

references

[1] RenataS Mendonza, DeniseNavia, IvonRDini, etal. SoupAmerican Spicer Mittes.

Newtownslocolics [J]. Jounalof Scene Science, 2011, 11 (5). 1-18.

[2] J. Gutiererer. TheThessavaGreenMitinAfrica. OneTorse Spices? (Acari. Tetranychi-dae) [J]. Experimental & Applicable Carel, 1987, 3. 163-168.

[3] Carlos Flechtman. Thecassava mitcomplex. taxonomy and difiction [J].

Proceedings Cascade Prospecting Worldshop, 1977, 11. 143-153.

[4] Wang Huifu, et al. Economic Entomology (Book 23, Tetranychusidae) [M]. Beijing. Science Press, 1981. 19-28, 88.

[5] Marx Pei, Shen Zhaopeng, Chen Xiwen, etc. Chinese agricultural mites [M]. Shanghai. Science and Technology Press, 1984. 6-94.

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