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SN/T 1135.11-2013Detection and identification of Polyscytalum pustulans (M.N.Owen et Wakef.)M.B.Ellis (English PDF)

马铃薯皮斑病菌检疫鉴定方法

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Issued by

General Administration of Customs

Level / Type

National · Recommended

Issue date

November 6, 2013

Implementation date

June 1, 2014

Scope

SN/T 1135.11-2013 is the English-translated version of 马铃薯皮斑病菌检疫鉴定方法.

SN/T 1135.11-2013 - SN/T 1135 This section provides the quarantine and identification of potato peel leaf spot pathogen. This section applies to quarantine and identification of potato tubers potato peel leaf spot pathogen.

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Document preview — SN/T 1135.11-2013

National Standard of the People's Republic of China

ICS: 65.020
Classification: B16

Issued by: General Administration of Customs

Foreword
1 Scope
2 Basic information of germs
3 PRINCIPLE OF THE METHOD
4 Apparatus and appliances
4.1 Instrument
4.2 Appliances
5 Reagents and culture medium
5.1 Reagents

Foreword

This section drafted in accordance with GB/T 1.1-2009 given rules.

This section proposed and managed by the National Certification and Accreditation Administration Committee.

This section drafted by. China Inspection and Quarantine Science Research Institute.

Drafters of this section. Wu Shan goods, Du Hongzhong, Yan Jin, Zhang Qiue.

Potato skin patch quarantine and identification of bacteria

1 Scope

SN/T 1135 This section provides the quarantine and identification of potato peel leaf spot pathogen.

This section applies to quarantine and identification of potato tubers potato peel leaf spot pathogen.

2 Basic information of germs

Chinese name. potato skin patch germs English name. Skinspotofpotato Scientific name. Polyscytalumpustulans (MNOwenetWakef.) MBElis Synonym. OosporapustulansM.N.Owen Taxonomic position. Fungi (Fungi), mitosis fungal spores (Mitosporicfungi), snake Mortierella (Polyscytalum).

Transmission. pathogen primary infection source is a tuber, spores by rain, irrigation close spread by seed or remote tape Soil disease spread.

For additional information potato peel leaf spot pathogen in Appendix A.

3 PRINCIPLE OF THE METHOD

Potato skin patch pathogen morphology and molecular characterization of identification basis.

4 Apparatus and appliances

4.1 Instrument

Microscope (with measurements), stereo microscope, clean benches, biological incubator, scales, autoclaves, desktop high-speed centrifugation Machine, pH meter, water bath, real-time PCR amplification.

4.2 Appliances

Beakers, flasks, graduated cylinder, test tubes, petri dishes, alcohol lamp, tweezers, scissors, scalpels, needle inoculation, slides, cover slips, plastic pestle Pipettes, pipette tips, centrifuge tubes, liquid nitrogen tank.

5 Reagents and culture medium

5.1 Reagents

Glucose, agar powder, liquid nitrogen, Tris-HCl, MgCl2, HCl, EDTA, NaOH, NaOAC, KCl, Tris, CTAB, TAE, no Aqueous ethanol (Ethanol), SYBRGreenI nucleic acid dyes, chloroform (Chloroform), isopropyl alcohol (Isopropanol), iso-amyl alcohol (Isoamyl alcohol), proteinase K, TaqDNA polymerase, dNTP, universal primers ITS5 and ITS4, real-time PCR primers PpustF1, PpustR2 and probe PpustPr1.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 8 pages — is available in the English PDF.

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