LY/T 3124-2019Guidelines for the conduct of tests for distinctness, uniformity and stability - Bambusa (Bambusa Retz. corr. Schreber) (English PDF)

3.1 Bamboo shoot emerging interval

The time from when a bamboo plant shoots to the next year.

3.2 Young stalks

Stalks not more than half a year old since the bamboo shoots were unearthed.

4.1 Test materials

4.1.1 The time, place and quantity and quality of plant materials required for testing shall be notified by the approving agency. Never test ground for materials submitted by a country or region, the applicant shall provide relevant documents such as customs, phytosanitary and other related matters in accordance with relevant regulations on entry and exit and transportation.

4.1.2 The test materials submitted shall be asexually propagated 1-year or 2-year-old plants, with 3 bamboo shoots associated with each plant.

Strong and strong shoots; if it is sexually propagating plants, one-year or two-year-old plants should be selected when the plant characteristics are stable.

4.1.3 The number of test materials submitted shall not be less than 10 plants.

4.1.4 The material of the new variety to be tested shall be a plant with normal growth without obvious pest and disease hazards.

4.1.5 Except for those materials approved or required by the approval authority, the submitted plant materials should not be subjected to any amount that affects the expression of traits.

Outside treatment. If it has been processed, provide details of the processing.

4.2 Test method

4.2.1 Test cycle and time

If the test conditions are met, test at least one shoot-out cycle.

4.2.2 Test location

The test location of the variety to be tested shall be selected in the test base and laboratory designated by the approval agency.

4.2.3 Test conditions

The test should be performed under the condition that the relevant characteristics of the tested species can be fully expressed.

Planted for more than two years.

4.2.4 Test Design

4.2.4.1 Ten new varieties to be tested shall be planted in the test area and planted in the same location and environmental conditions as standard varieties and similar varieties.

4.2.4.2 If some parts of the plant need to be taken as samples for the test, the sample collection must not affect the entire shoot emergence cycle of the test plant Observation.

4.2.4.3 Unless otherwise stated, all observations shall be made on 10 plants or material taken from the same part of 10 plants.

4.2.5 Test methods for similar characteristics

4.2.5.1 Typical characteristics of stalks, branches, leaves, stalks, etc.

Stalk. When the high growth of Hsinchu is basically stopped, the 4th to 7th sections of the stalk base of a normal growing plant are upwards as the test material for bamboo stalk characteristics;

For the deformed stalk, the deformed part is used as the test material.

Branches. The middle or lower branches of the plants that were born that year are used as the test materials for the branch traits. More than 3 plants should be selected for the test.

Leaves. Within one month after the new leaves are fully unfolded, select the middle leaves with leaf twigs on the middle plate or lower branches of the current year's plants as the leafiness Test material. For leaf character test, more than 10 samples should be selected at random, and the average value should be taken.

Stalk clams. During the growth period of bamboo shoots to young bamboos, stalk clams at the 4th to 7th sections of the stalk base are selected as the test material.

4.2.6 Test criteria for individual characteristics

4.2.6.1 Leaf. Leaf base shape (Table A.1 Characteristic No. 14 in Appendix A) Characteristics

Wedge, wide wedge, oval.

4.2.6.2 Condylar sheath. apex shape (Table A.1 in Appendix A. Characteristic number 17) Characteristics Depressed, truncated, raised.

4.2.6.3 Ears. Shape (Table A.1 in Appendix A. Characteristic Characteristic Number 23) Features

Oval, long ovate, sickle-shaped.

4.2.6.4 Sepals. Shape (Table A.1 in Appendix A. Character No. 27) Characteristic

Wide triangle,, lanceolate.

4.2.6.5 Sepals. Apex shape (see Appendix A, Table A.1 Characteristic Characteristic Number 32).

Long pointed, tapered, and pointed.

5.1 Specificity

If the difference in traits satisfies a constant difference and a significant difference, it is considered to be specific.

5.1.1 Constant difference

If the difference between the new variety to be tested and the similar variety is very clear, only one test of the shoot cycle is needed. In some cases when the difference between the tested varieties and similar varieties is unclear, at least two or more test cycles are needed.

5.1.2 Significant differences

Specific evaluation of quality traits. As long as there is a significant difference in one trait between the tested variety and a similar variety, it can be determined that the variety has specific characteristics opposite sex.

Quantitative trait-specific evaluation. at least two traits of the tested variety and similar varieties are significantly different, or two generations of one trait the difference in codes can determine that the breed is specific.

Specific evaluation of false quality traits. at least two traits of the tested variety and similar varieties are different, or two different traits of one trait Differences in consecutive codes can be used to determine the specificity of the breed.

5.2 Consistency

Consistency is judged by the heterotypic strain method. According to the 1% population standard and 95% reliability probability, the maximum allowable number of heterotypic plants among 10 observed plants the allowed value is 1.

5.3 Stability

5.3.1 The variety to be tested meets the specificity and consistency requirements in the test, and the variety can be considered to have stability.

5.3.1.1 In special circumstances or in doubt, it is necessary to pass a new test cycle or to provide a new test by the applicant Materials to test whether they express the same characteristics as the test materials provided previously.

6.1 Variety group description

According to the characteristics of the group, determine the grouping of the tested varieties, and select similar varieties to be included in the specific growth test.

6.2 Grouping characteristics

6.2.1 Bamboo plants. branch thorns (Table A.1 Characteristic number 8).

6.2.2 sheath base width/箨 leaf top width (Table A.1 trait characteristic number 33).

6.2.3 Sepals. Stretched state (Table A.1 Characteristic feature number 28).

7.1 Feature types

7.1.1 Asterisk characteristics (characters marked with "(*)" in Table A.1). refer to the use of new varieties for the purpose of coordinating the description of the traits during review.

Important variety characteristics, all "asterisk characteristics" should be tested when performing the DUS test.

7.1.2 Plus feature (character marked with "(+)" in Table A.1). refers to the description of the characteristic table in Table A.1 Characteristics (see A.2).

7.2 Expression status and code

In Table A.1, the trait feature description has clearly given the standard definition of the trait expression status for each trait.

Line description and analysis and comparison, each expression state has a corresponding numeric code.

7.3 Expression types

GB/T 19557.1-2004 provides the expression types of trait characteristics. qualitative traits, quantitative traits, and pseudo-quality traits.

7.4 Standard varieties

Varieties used to accurately and vividly demonstrate the expression status of a certain trait (especially quantitative traits).

7.5 Symbol description

The symbols that appear in Table A.1 of Appendix A are as follows.

(*). Asterisk feature, see 7.1.1;

(). Plus sign feature, see 7.1.2;

QL. quality traits, see 7.3;

QN. quantitative traits, see 7.3;

PQ. false quality traits, see 7.3;

MG. a single record from a single measurement of a group of plants or plant parts;

MS. Multiple records are obtained for a certain number of plants or plant parts respectively to obtain multiple records;

VG. a single visual inspection of a group of plants or plant parts to obtain a single record;

VS. Obtain multiple records by visual inspection for a certain number of plants or plant parts;

(A). Corresponds to 4.2.5.1;

(B), (c), (d), (e), (f), (g). Corresponding to 4.2.6.1, 4.2.6.2, 4.2.6.3, 4.2.6.4, 4.2.6.6.

Appendix A

(Normative appendix) Variety Traits A.1 Characteristic table

Table A.1

Serial number and

nature

test

method

Trait Feature Description Standard Variety Generation Chinese name scientific name QL VG (a) New stalk. Festival White powder ring Without bamboo B.blumeana JAet JH Schult.F.

B.boniopsis 'Yello-Stripe' 3 Strong filial piety B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

New stalk. Internode White powder B.ventricosa McClure 1 Weak bamboo B.blumeana JAet JH Schult.F.

B.cerosisssima McClure 5 (*) PQ MS new stalk. coat Type fluffy filial bamboo B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

B.cerosisssima McClure 3 (*) QL VG (a) New stalk. ring Bamboo without filial piety B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

There is single bamboo B.cerosisssima McClure 2 (*) PQ VG (a) New stalk. color Green filial bamboo B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

Green, branch side

yellow

B.albo-lineata LCChia 3 Green and yellow stripes Golden jasper bamboo B.vulgaris var.striata Yellow, branched side green Yellow bamboo B. eutuldoides var.

viridivittata

MS stalk. internode length Short Buddha belly B.vulgaris cv.wamin 3 Zhongxiaoshun Bamboo B.multiplex (Lour.) Raeuschel ex JAet JHSchult.

B.cerosisssima McClure 7

Table A.1 (continued)

Serial number and

nature

test

method

Trait Feature Description Standard varieties Chinese name scientific name QN MS Bamboo. DBH Small filial bamboo B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

Medium Indica

B.blumeana JAet JHSchult.

Large Indica B. arundinacea (Retz.) Willd. 3 QL VG (a) Bamboo. B.vulgaris var.striata 1 There is bamboo B.blumeana JAet JH Schult.F.

MS Bamboo. First

Branch height

Low Bamboo

B. subaequalis HLFung et CYSia Middle gold jasper bamboo B.vulgaris var.striata 2 B.cerosisssima McClure 3 QL VG stalk. morphology Normal filial bamboo B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

B.ventricosa McClure 9 QN VS leaves. every twig Number of leaves Lesser bamboo B.blumeana JAet JH Schult.F.

Middle gold jasper bamboo B.vulgaris var.striata 2 B.ventricosa McClure 3 QN MS leaf. length Short leaf lyre B.multiplex (Lour.) Raeuschel ex JA et J H. Schult var multiplex 'Stripestem Fernleaf' RA Young Zhongxiaoshun Bamboo B.multiplex (Lour.) Raeuschel ex JAet JHSchult.

Long gold jasper bamboo B.vulgaris var.striata 3 QL VS (a) Leaves. dorsal quilt Without bamboo B.blumeana JAet JH Schult.F.

B.pervariabilis McClure 9 () QN VS (b) Leaf. leaf base B.nutans Wall.ex Munro 1 B.ventricosa McClure 2 Olympia lobata B.multiplex (Lour.) Raeuschel ex JA et J H. Schult var multiplex 'Stripestem Fernleaf' RA Young

Table A.1 (continued)

Serial number and

nature

test

method

Trait Feature Description Standard varieties Chinese name scientific name (*) QL VS (a) Scabbard. coat Bamboo without filial piety B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

B.cerosisssima McClure 3 B.cerosisssima McClure 5 QN VG (a) Scabbard. white B.ventricosa McClure 1 Phyllostachys pubescens B.multiplex (Lour.) Raeuschel ex JA et J H. Schult var multiplex 'Stripestem Fernleaf' RA Young Strong single bamboo B.cerosisssima McClure 3 (*) () QN MS (c) Scabbard. apex shape Concave single bamboo B.papillata QHDai 1 Prunus officinalis B.blumeana JAet JHSchult.

Raised leaflet

B.multiplex (Lour.) Raeuschel ex JA et J H. Schult var multiplex 'Stripestem Fernleaf '' RA Young (*) QL MS (a) Scabbard. edge Coat B.nutans Wall.ex Munro 1 Filial Bamboo B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

Sheath. shorter than B.ventricosa McClure 1 Equal to internode Indica B. arundinacea (Retz.) Willd. 2 B.vulgaris 'Wamin' 3 (*) QL VS (a) Sheath. Motley Striped filial piety bamboo B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

B.ventricosa McClure 3 B.pervariabilis McClure 5 (*) QL MS ear Bamboo without filial piety B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

B.pervariabilis McClure 5

Table A.1 (continued)

Serial number and

nature

test

method

Trait Feature Description Standard Variety Generation Chinese name scientific name QN VS (a) Er ear. Yes Nearly equal Zhuzhu B.blumeana JAet JH Schult.F.

No. B. pervariabilis McClure 3 (*) () QN VS (d) Ears. B.ventricosa McClure 1 B. pervariabilis McClure 2 Sickle-like bamboo B.blumeana JAet JH Schult.F.

MS Ears. Hair

Developed or not

B.ventricosa McClure 1 it is Burmese bamboo B.burmanica McClure 3 QL VG ear. with Connected or not no gold jasper bamboo B.vulgaris var.striata 1 it is B.ventricosa McClure 9 (*) QL VS (a) Ears. Downward or not no bamboo B.blumeana JAet JH Schult.F.

Yes Bamboo B.nutans Wall.ex Munro 3 (*) () QN VG (e) Sepals. Wide-angled triangular braised bamboo B.pervariabilis McClure 1 Triangle filial bamboo B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

B.ventricosa McClure 7 (*) QN VS (a) Sepals. Stretching status B.ventricosa McClure 1 Reversed Pink Bamboo B.chungii McClure 3 (*) () QN VS Sepals. Wrinkled Filial piety B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

It is yellow-striped mom bamboo B.boniopsis 'Yello-Stripe' 3 QL VS (a) Sepals. Back Coat or not B.ventricosa McClure 1 it is Burmese bamboo B.burmanica McClure 5 QL VS (a) Sepals. ventral Coat or not no Mom Bamboo B.boniopsis McClure 1 it is B. rundinacea (Retz.) Willd. 5 () QN MS (f) Sepals. Apex Shaped long pointed filial bamboo B.multiplex (Lour.) Raeuschel ex JAet JH Schult.

B.ventricosa McClure 2 Echidna acuminata B.blumeana JAet JH Schult.F.

Table A.1 (continued)

Serial number and

nature

test

method

Trait Feature Description Standard Variety Generation Chinese name scientific name QN VS cymbal base width/箨 Sheath top width Less than 1/2 Xuelin Dianthus B. xueliniana RSLin Zheng 1/2 ~ 2/3 B.ventricosa McClure 3 About 1 filial bamboo B.multiplex (Lour.) Raeuschel ex JAet JHSchult.

Sepal length/箨

Sheath length < 1

B.multiplex (Lour.)

Raeuschel ex JAet JH Schult.

1 B. mutabilis McClure 2 > 1 B.boniopsis 'Yello-Stripe' 3 A.2 Characteristic table illustration A.2.1 Characteristic Table No. 14, Leaf. Leaf base shape Wedge wide wedge oval 1. The base of the leaf gradually narrows to the petiole; 2. The base of the leaf narrows rapidly to the petiole; 3. The base of the leaf is wider, shaped like chicken eggs.

A.2.2 Characteristic table No. 17, scabbard. apex shape Concave truncated protrusion 1. Recessed H > 1/15L; 3. Intersection H<=1/15L; 5. Projection H > 15L.

A.2.3 Characteristic table No. 23, Er ear. shape Ovate 1. The ear-to-width ratio is less than 1.2; 2. the ear-to-width ratio is 1.3 to 2.0; 3. the ear-to-width ratio is greater than 2.0.

A.2.4 Characteristic Table No. 27, Sepal. Shape Wide triangle lanceolate 1.H/L < 1.0; 3.H/L = 1.0 ~ 3.9; 5.H/L>=4.0.

Appendix B

(Informative appendix) Technical Questionnaire Number (not required for applicants) 1. Name of the variety for which registration is requested (please specify Chinese name and scientific name).

2. Applicant Information Applicant. Co-applicant.

address.

Zip code. Phone. Fax. E-mail.

3. Origin of the variety.

Breed finder. Discovery date. Breeder. Breeding time.

Cross breeding. (female) (female) × (male) (parent) Live breeding. (female) (female) Other breeding routes.

Summary of the breeding process.

4. Main characteristics (The numbers in the parentheses in the first column are the serial numbers of the trait characteristics in Table A.1 of Appendix A. Please mark "sqrt" in [] after the corresponding characteristic code) 4.1 (1) Underground stem. stomatal channel 1 without [], 9 with [] 4.2 (2) New stalk. white powder ring under the knot 1 no or extremely inconspicuous [], 2 moderate [], 3 obvious [] 4.3 (3) New stalks. quilt loop coat loop 1 without [], 2 fine loops [], 3 developed loops [] 4.4 (4) New stalks. internodes white powder 1 no [], 2 slightly white powder [], 3 obviously thick white powder [] 4.5 (5) New stalk. internode coat 1 without [], 2 sparse coat [], 3 dense coat [] 4.6 (7) Stalk. color 1 green [], 3 green, groove yellow [], 5 yellow-green stripes meet [], 7 yellow, groove green Color [], 9 yellow, with occasional green stripes [] 4.7 (11) Stalk. internode shape 1 normal cylindrical [], 2 part stalk internode deformity [], 3 all stalk internode deformity [] 4.8 (14) Stalk. internodes are spotted 1 not spotted [], 2 grooved only spotted [], 3 all spotted or spotted [] 4.9 (17) Stalk knots. intra-node length 1 short [], 2 medium [], 3 long [] 4.10 (20) Stem nodes. Comparison of two ring heights 1 Stem ring is lower than cymbals [], 2 equal heights [], 3 culm rings are higher than cymbals [] 4.11 (21) Leaf (medium plate). The number of leaves per branch is 1 less [], 2 medium [], 3 more [] 4.12 (24) Leaf. The number of leaf veins is 1 less [], 2 medium [], 3 more [] 4.13 (30) Leaf. Ye ear 1 without [], 2 weak [], 3 developed [] 4.14 (31) Leaf. Hairy 1 without [], 2 short [], 3 developed [] 4.15 (37) Sheath. Coat 1 without [], 3 sparse [], 5 medium [], 7 dense [], 9 very dense [] 4.16 (39) sheath. base hair loop 1 without [], 2 fine hair loop [], 3 obvious hair loop [] 4.17 (40) Sheath. white powder 1 without [], 2 thin white powder [], 3 obviously white powder [] 4.18 (41) sheath. number of spots 1 none [], 3 sparse [], 5 medium [], 7 dense [], 9 very dense [] 4.19 (42) Scabbard sheath. apex shape 1 markedly concave [], 3 slightly concave [], 5 truncated or nearly truncated [], 7 slightly convex [], 9 strongly convex from [] 4.20 (43) Sheath. marginal coat 1 without [], 3 coat < 1/3 [], 5 coat 1/3-2/3 [], 7 coat> 2/3 [], 9 whole Coat [] 4.21 (44) sheath. shorter than internode 1 than internode [2], 2 is nearly equal to internode [3], 3 is longer than internode [1] 4.22 (45) Ears. size 1 none or very insignificant [], 3 weak [], 5 moderate [], 7 developed [], 9 extremely developed [] 4.23 (46) Ears. color 1 green [], 2 hazel [], 3 brown or purple [] 4.24 (47) Ear. Shape 1 ovate [], 2 long ovate [], 3 sickle-shaped [] 4.25 (48) Ears. growth position 1 separates from sepals [], 2 bases are connected to sepals [], 3 whole and sepals [] 4.26 (50) Hair. color 1 green [], 2 hazel [], 3 brown or purple [] 4.27 (51) Hair. morphology 1 straight [], 2 slightly curved [], 3 strongly curved [] 4.28 (52) Sepals. shape 1 triangle [], 3 long triangles [], 5 lanceolate [], 7 long lanceolate [], 9 stripe [] 4.29 (53) Sepals. base width 1 is nearly equal to the top of the scabbard [], 2 is the top of the scabbard 2/3-1/2, 3 is less than the top of the scabbard 1/2 [] 4.30 (54) Sepals. base shape 1 trumpet shape [], 2 straight tube shape [], 3 oval shape [] 4.31 (55) Sepals. growing state 1 erect or nearly erect [], 2 flat [], 3 inverted [] 4.32 (56) Sepals. whether wrinkles 1 straight [], 3 apex 1/3 fold [], 5 apex 1/3-1/2 fold [], 7 apex 1/2-2/3 Wrinkles [], 9 all wrinkles [] 4.33 (57) Sepals. coat 1 without [], 9 coat [] 4.34 (58) Sepals. color 1 green [], 2 green with purple [], 3 color [] 4.35 (60) Tongue. height 1 low [], 2 medium [], 3 high [] 4.36 (61) Tongue. marginal eyelashes 1 cilia [], 2 fine hairs [], 3 thick hairs [] 4.37 (62) Tongue. color 1 light white [], 2 light brown [], 3 purple or brown [] 4.38 (63) Tongue. whether it is delayed 1 not delayed [], 2 slightly delayed [], 3 strongly delayed [] 4.39 (64) Tongue. apex shape 1 significantly concave [], 3 slightly concave [], 5 truncated or nearly truncated [], 7 slightly convex [], 9 significantly convex from [] 5. Comparison information of similar varieties Variety names similar to this variety.

Typical differences from similar varieties.

6. Summary of variety characteristics (detailed description according to Table A.1 in Table A.1) 7. Additional information (ability to distinguish traits of varieties, etc.)

7.1 Stress resistance and adaptability (drought resistance, cold resistance, waterlogging resistance, salt and alkali resistance, to pests and diseases)

7.2 Reproduction points.

7.3 Main points of cultivation management.

7.4 Other information