LY/T 3123-2019Guidelines for the conduct of tests for distinctness,uniformity and stability(DUS) -- Wattle(Acacia Mill.)(Phyllodium Type) (English PDF)

4.1 Test materials

4.1.1 The time, place and quantity and quality of plant materials required for testing shall be notified by the approving agency. Never test Applicants must provide relevant documents such as customs, plant quarantine, etc. in accordance with relevant regulations on entry, exit, and transportation Pieces.

4.1.2 The test materials are at least biennial plants that have passed asexual reproduction.

4.1.3 The number of test materials shall not be less than 15 plants.

4.1.4 The test materials must be plants that are not infected by pests and normal growth.

4.1.5 Except as permitted or required by the approving agency to process the test materials, the submitted test materials should not be subjected to any form that affects the traits Up to the processing. If it has been processed, details of the processing must be provided.

4.1.6 The variety right applicant should submit a technical questionnaire when applying, see Appendix B.

4.2 Test method

4.2.1 Test cycle and time

Subject to test conditions, test at least one growth cycle.

4.2.2 Test location

Testing should be performed at test sites and laboratories designated by the approving agency.

4.2.3 Test conditions

The test should be performed under the condition that the relevant characteristics of the tested species can be fully expressed, and the selected test materials should be at least at the test site.

Point colonization over 2a years.

4.2.4 Test Design

4.2.4.1 Each test should be based on 15 plants. The variety to be tested should be planted in the same location as the standard variety and similar varieties.

And environmental conditions.

4.2.4.2 If some parts of the plant need to be taken as samples for the test, the sample collection shall not affect the whole growth cycle of the test plant.

Observe.

4.2.5 Test methods for similar traits

(A) Measurement of characteristics of typical plants, trunks, current year branches, petioles, flowers, inflorescences, pods, seeds, etc.

Sample material observation method Plant. Observe when the plant enters the dormant period in autumn or winter.

Stem. Observe plants at least 5 years old in autumn or winter.

Current year branches. In summer or autumn, select the current year branches in the middle and upper part of the canopy of the test plant (5 branches per plant) as a test material for the characteristics of the branches that year.

Petiole. After the leaves mature in summer or autumn, select the middle leaves of the branches in the middle and upper part of the crown of the test plant on the sunny side of the year the sheet serves as a test material for the characteristics of the petiole.

Flowers. Enter the full bloom period, and select the small flowers that have been fully opened in the inflorescences of the upper and middle branches of the test plant sunny side as flower features Test materials.

Inflorescence. Enter the full bloom period, and select the inflorescence of the middle and upper branches of the test plant sunny side as the test material for flower inflorescence characteristics.

Fruit. After the pods are mature, the fruiting branches of the upper and middle crowns of the test plants are selected as test materials for fruit characteristics.

Seeds. After the pods have matured, the pods at the initial stage of cracking on the fruiting branches of the test plant sunny side canopy are selected for observation Test material for seed characteristics.

4.2.6 Test methods for individual traits

4.2.6.1 The anthocyanin coloration of the current branch should be observed in spring.

4.2.6.2 Petiole color is the observation of petiole color during the leafing stage.

4.2.7 Additional tests

New hybrid varieties obtained through natural pollination or artificial pollination, if the stability test is in doubt, special characteristics of their parents should be added.

Heterosexuality, consistency and stability testing.

5.1 Specificity

If the difference in traits satisfies a constant difference and a significant difference, it is considered to be specific.

5.1.1 Constant difference

If the difference between the new variety to be tested and the similar variety is very clear, only one growth cycle test is required. In some cases If the difference between the new variety to be tested and the similar variety is unclear due to the influence of environmental factors, at least two or more growth cycles are required.

test.

5.1.2 Significant differences

Specific evaluation of quality traits. As long as there is a difference in traits between the new variety to be tested and similar varieties, it can be determined that the variety has Specificity.

Quantitative trait specificity evaluation. at least two traits of the tested new variety and similar varieties are different, or two of one trait are different Differences in codes (see Table A.1) can be used to determine that the breed is specific.

Specific evaluation of false quality traits. there are at least 2 differences in traits between the tested new variety and similar varieties, or two differences in one trait the difference between two discontinuous codes can determine that the breed is specific.

5.2 Consistency

Consistency is judged by the heterotypic strain method. According to the 1% population standard and 95% reliability probability, the most heterogeneous plants among 15 observed plants the maximum allowed value is 1.

5.3 Stability

5.3.1 The applied variety meets the specificity and consistency requirements in the test, and the variety can be considered to have stability.

5.3.2 In special circumstances or in doubt, it is necessary to pass a test of a growth cycle again or to provide new test materials by the applicant, Test whether it expresses the same characteristics as the test material provided previously.

6.1 Variety group description

Determine the grouping of new varieties to be tested based on the grouping traits, and select similar varieties to be included in the specific growth test.

6.2 Grouping Traits

6.2.1 Plant. Plant shape (character number 1 in Table A.1)

6.2.2 Plant. Single stem (Table A.1 Character No. 5)

6.2.3 Current year branch. leaf-shaped stalk inoculation method (Table A.1 Character No. 14)

6.2.4 Petioles. Number of main veins (Table A.1 Character No. 23)

6.2.5 Inflorescence. Type (Table A.1 Character No. 24)

7.1 Character types

7.1.1 Asterisk traits (characters marked with "(*)" in Table A.1) refer to the traits used in the review of new varieties for the purpose of harmonizing and unifying traits Important variety traits, all "asterisk traits" must be tested when performing the DUS test.

7.1.2 Plus traits (characters marked "()" in Table A.1) refer to the traits illustrated in the table of characteristics of Table A.1 (See Table A.2).

7.2 Expression status and code

In Table A.1, the trait characteristics description has clearly given the standard definition of the expression status of each trait.

Line description and analysis and comparison, each expression state has a corresponding numeric code.

7.3 Expression types

GB/T 19557.1-2004 has provided the expression types of traits. qualitative traits, quantitative traits and pseudo-quality traits.

release.

7.4 Standard varieties

Varieties used to accurately and vividly demonstrate the expression status of a certain trait (especially quantitative traits).

7.5 Symbol description

The symbols that appear in Table A.1 of Appendix A are as follows.

(*). Asterisk character, see 7.1.1;

(). Plus character, see 7.1.2;

QL. quality traits, see 7.3;

QN. quantitative traits, see 7.3;

PQ. false quality traits, see 7.3;

MG. It is a single measurement for a group of plants or plant parts to obtain a single record;

MS. multiple records are obtained for a certain number of plants or plant parts respectively;

VG. It is a single visual inspection for a group of plants or plant parts to obtain a single record;

VS. multiple records are obtained by visual inspection of a certain number of plants or plant parts;

(a) See 4.2.5 for the test method;

(B) See 4.2.6.1 for test methods;

(c) For the test method, see 4.2.6.2.

Appendix A

(Normative appendix) Variety Traits A.1 Characteristic table

Table A.1 Characteristic table

Serial number and

nature

test

method

Trait Feature Description Standard Variety Generation Chinese name scientific name (*) QL VS/VG (A) Plant. Plant shape upright Creeping QN VG/VS (a) Plant. Height (Only for uprights Species) 'Mini Cog' 'Lime Magik' () PQ VG/VS (a)

Figure A.1

Plant. Crown

triangle

Semicircle

Round

Oval

VG/VS

Figure A.2

Plant. first branch extension Show attitude upright Half-open Opening Sagging 'Crimson Blush' 'Lime Magik' (*) QL VS (a) Plant. Single stem () PQ VG/VS (a)

Figure A.3

Trunk. Bark

smooth

Lump

Longitudinal split

Figure A.4

Trunk. degree of bending (Only for dry products Species) None or near none QL VS (a) Branch. thorn without QN VG/MG (a) Branch. Primary branch thickness (Only for dry products Species) Martha Cross No. 1 Martha Cross 5 Acacia 'Hybrid-1' Acacia 'Hybrid-5'