LY/T 3122-2019Guidelines for the conduct of tests for distinctness, uniformity and stability (DUS)--Creeper (Parthnocissus Planch) (English PDF)

4.1 Test materials

4.1.1 The time, place and quantity and quality of plant materials required for testing shall be notified by the approving agency. From non-test country Applicants must provide relevant documents such as customs, plant quarantine, etc. in accordance with relevant regulations on entry, exit, and transportation.

4.1.2 The test material is asexually propagating self-rooted seedlings of the same age over 2a.

4.1.3 The number of test materials shall not be less than 15 plants.

4.1.4 The test materials must be plants that are not infected by pests and normal growth.

4.1.5 Except that the testing authority allows or requires the testing materials to be processed, the submitted testing materials should not be subjected to any influence on the expression of traits.

deal with. If it has been processed, details of the processing must be provided.

4.1.6 The variety right applicant should submit a technical questionnaire when applying, see Appendix B.

4.2 Test method

4.2.1 Test cycle and time

Subject to test conditions, test at least one growth cycle.

4.2.2 Test location

Testing should be performed at test sites and laboratories designated by the approving agency.

4.2.3 Test conditions

The test should be performed under the condition that the relevant characteristics of the tested species can be fully expressed.

Plant 2a or more.

4.2.4 Test Design

4.2.4.1 Each test shall be based on 15 plants. The test variety shall be planted in the same location and environment as the standard variety and similar varieties Environmental conditions.

4.2.4.2 If some parts of the plant need to be taken as samples for the test, the sample collection must not affect the observation of the entire growth cycle of the test plant Measurement. If there is an impact, a sufficient number of plants should be planted to ensure that the number of remaining plants can still meet other requirements after testing and sampling during growth.

Observation needs.

4.2.4.3 Unless otherwise stated, all observations shall be made on 10 plants or material taken from the same part of 10 plants. Observation for organs or parts of plants, one sample should be taken from each plant. Population observation traits (VG, MG) should observe the entire community or stipulate large Small mixed samples.

4.2.5 Test methods for similar traits

Visual inspection of typical branches, leaves, flowers and fruits.

Young leaves. During the flowering period, take the 2 ~ 4 unfolded young leaves of the new shoot tip of the year to observe.

Mature leaves. From the young fruit stage to the fruit turning stage, select the leaves on sections 5-9 above the base of the current year's branches to observe.

New shoots. From the young fruit stage to the fruit turning stage, select the middle of the new shoots for observation.

Mature branches. In the deciduous period, take the middle of the current year branches for observation.

Inflorescence. Select the complete inflorescence from the beginning to the full bloom period for observation.

4.2.6 Test methods for individual traits

4.2.6.1 Branches. Observation of twigs (Table A.1 Characteristic number 7) shall be selected for observations of biennials or more.

4.2.6.2 Tendril (Table A.1 Characteristic characteristics No. 8-9). During fruit setting to fruit turning period, select the 2-3 tip of the tip but not attach it Observation of tendril in contact with objects.

4.2.6.3. Leaf discoloration (Table A.1 Characteristic characteristics No. 28-29). Observe from leaf discoloration period to deciduous period.

4.2.6.4 Phenotypic characteristics (Table A.1 Character No. 30-34). about 5% of bud eyes are in the germination stage; about 5% of buds are open it is the beginning of flowering period; when 50% of the fruit is blue-purple, it is the fruit discoloration period; about 50% of the leaves are discolored when it is autumn; about 50% of the leaves Normal fall-off period is deciduous.

4.2.7 Additional tests

For new hybrid varieties obtained by natural or artificial pollination, if there is any doubt about the stability test, the specificity of their parents, Consistency and stability testing.

5.1 Specificity

If the difference in traits satisfies a constant difference and a significant difference, it is considered to be specific.

5.1.1 Constant difference

If the difference between the new variety to be tested and the similar variety is very clear, only one growth cycle test is required. In some cases due to circumstances when the influence of factors makes the difference between the new variety to be tested and the similar variety unclear, at least two or more growth cycle tests are required.

5.1.2 Significant differences

Specific evaluation of quality traits. As long as there is a difference in traits between the new variety to be tested and similar varieties, it can be determined that the variety has specific characteristics.

opposite sex.

Quantitative trait-specific evaluation. at least two traits of the tested new variety and similar varieties are different, or two generations of one trait the differences in the codes (see Table A.1) can be used to determine the specificity of the breed.

Specific evaluation of false quality traits. there are at least two different traits between the new variety and similar varieties to be tested, or two of one trait Differences in discontinuous codes can determine that the breed is specific.

5.2 Consistency

Consistency is judged by the heterotypic strain method. According to the 1% population standard and 95% reliability probability, the largest number of heterotypic plants among the 10 observed plants the allowed value is 1.

5.3 Stability

5.3.1 The applied variety meets the specificity and consistency requirements in the test, and the variety can be considered to have stability.

5.3.2 Under special circumstances or in doubt, the test needs to be performed by testing a growth cycle again, or by providing new test materials by the applicant.

Test whether it expresses the same characteristics as the test materials provided previously.

6.1 Variety group description

Determine the grouping of new varieties to be tested based on the grouping traits, and select similar varieties to be included in the specific growth test.

6.2 Grouping Traits

6.2.1 Leaf. Leaf type (Table A.1 Characteristic number 10).

6.2.2 Tendril. Top shape (Table A.1 Characteristic number 8)

7.1 Character types

7.1.1 Asterisk traits (characters marked with "(*)" in Table A.1) refer to the important traits used to coordinate the description of traits during the review of new varieties.

For the desired variety traits, all "asterisk traits" must be tested when performing the DUS test.

7.1.2 Plus traits (characters marked with "()" in Table A.1) refer to the traits that are illustrated in the table of trait characteristics in Table A.1 (see

Table A.2).

7.2 Expression status and code

Table A.1 has a standard definition of the expression status of each trait.

Report and analyze and compare, each expression state has a corresponding numerical code.

7.3 Expression types

GB/T 19557.1-2004 has provided expression types of traits. qualitative traits, quantitative traits, and pseudo-quality traits.

7.4 Standard varieties

Varieties used to accurately and vividly demonstrate the expression status of a certain trait (especially quantitative traits).

7.5 Symbol description

The symbols that appear in Table A.1 of Appendix A are as follows.

(*). Asterisk character, see 7.1.1;

(). Plus character, see 7.1.2;

QL. quality traits, see 7.3;

QN. quantitative traits, see 7.3;

PQ. false quality traits, see 7.3;

MG. It is a single measurement for a group of plants or plant parts to obtain a single record;

MS. multiple records are obtained for a certain number of plants or plant parts respectively;

VG. It is a single visual inspection for a group of plants or plant parts to obtain a single record;

VS. multiple records are obtained by visual inspection of a certain number of plants or plant parts;

(a) See 4.2.5 for the test method;

(b) For the test method, see 4.2.6.

Appendix A

(Normative appendix) Variety Traits A.1 Characteristic table

Table A.1 Characteristic table

Serial number and

nature

test

method

Traits

description

Standard Variety Generation Chinese name scientific name (*) PQ VG (a) New shoot. Central colour green Green with pink powder red Red wine Dijin 'Cuiyu' 'Jindai' P.tricuspidata P. laetevirens 'Cuiyu' P.henryana.'Jindai ' (*) QL VG (a) New tip. edgeless (*) QL VG (a) New shoot. crosscut Face shape Round or oblate Approximately square PQ VG (a) Annual branches.

colour

Grey-green

Grey-brown

Tan

'Cuiyu'

Dijin

'Plus cited number 1' P. laetevirens 'Cuiyu' P.tricuspidata P.quinquefolia 'Jiayin 1' (*) QL VG (a) Annual branches.

Pitting

MG/MS

Annual branches.

Internode length

Dijin

'Jindai'

'Plus cited number 1' P.tricuspidata P.henryana 'Jindai' P.quinquefolia 'Jiayin 1' (*) () QL VG (b) Second year born Upper branch. plug wings (*) () QL VG (b) Tendril. when tender Top shape Ball shape hook QN MG/MS (b) Tendril. Length Dijin 'Plus cited number 1' 'Jindai' P.tricuspidata P.quinquefolia 'Jiayin 1' P.henryana 'Jindai' (*) QL VG (a) Leaf. Type Monophylla winged brocade P.suberosa 1 Single leaf dominant Type leaf, plant base 3 leaflets compound leaves P.tricuspidata Ordinary bi-type leaves, 3 small on short branches Leaves compound leaves, long branches Often single leaf Polygonum heterophylla P.dalzielii 3 small leaf compound leaf clover trifoliate P. semicordata

5 P.quinquefolia 'Jiayin 1' (*) QL VG (a) Leaf. single leaf Number of cracks 3 crack 5 crack and above (*) QL VG (a) Leaf. leaf light (*) QN VG (a) Leaves. on young leaves Surface cyanine Glycoside coloring 'Cuiyu' 'Plus cited number 1' 'Jindai' P. laetevirens 'Cuiyu' P.quinquefolia 'Jiayin 1' P.henryana 'Jindai' QN VG (a) Leaves. on young leaves Surface fluff 'Jindai' Dijin 'Cuiyu' P.henryana 'Jindai' P.tricuspidata P. laetevirens 'Cuiyu' (*) QN VG (a) Leaf. upper surface Bulge degree None or weak 'Jindai' 'Plus cited number 1' 'Cuiyu' P.henryana 'Jindai' P.quinquefolia 'Jiayin 1' P. laetevirens 'Cuiyu' QN MS (a) Leaf. Midvein length Dijin 'Jindai' 'Cuiyu' P.tricuspidata P.henryana 'Jindai' P. laetevirens 'Cuiyu' () PQ VG (a) Ye. Central small Leaf shape Oval Wide oval Obovate 'Plus cited number 1' 'Cuiyu' P.quinquefolia 'Jiayin 1' P. laetevirens 'Cuiyu' () PQ VG (a) Ye. Central small Leaf base Narrow wedge Wedge Obtuse 'Cuiyu' 'Plus cited number 1' P. laetevirens 'Cuiyu' P.quinquefolia 'Jiayin 1' () PQ VG (a) Leaves. edge wavy Blunt teeth Jagged Heavy sawtooth Diloba heterophylla Trefoil Dijin P.dalzielii P.semicordat P.tricuspidata QN VG (a) Leaf. upper surface Coat Dijin 'Cuiyu' P.tricuspidata P. laetevirens 'Cuiyu' PQ (*) VG (a) Leaf. mature leaf Film color Light green 'Plus cited number 1' 'Jindai' 'Fenway Park' P.quinquefolia 'Jiayin 1' P.henryana 'Jindai' P.tricuspidata 'Fenway Park' QL (*) VG (a) Leaf. mature leaf Multicolor PQ (*) VG (a) Leaf. mature leaf Slice color vein other 'Jindai' P.henryana 'Jindai' QL (*) VG (a) Leaves. up and down Face color is no the same 'Cuiyu' 'Jindai' P. laetevirens 'Cuiyu' P.henryana 'Jindai' QN (*) VG (a) Leaf. lower surface Leaf vein flower Cyanoside coloring degree Very strong 'Cuiyu' 'Jindai' Trefoil Diphtheria P. laetevirens 'Cuiyu' P.henryana 'Jindai' P.semicordat P.suberosa (*) QN VG (a) Leaf. lower surface Leaf veins Glycoside coloring process None or very weak Very strong 'Cuiyu' 'Plus cited number 1' Trefoil 'Jindai' P. laetevirens 'Cuiyu' P.quinquefolia 'Jiayin 1' P.semicordat P.henryana 'Jindai' (*) QL VG (b) Ye. Growth period Whether changed color* PQ (*) VG/VS (b) Leaves. autumn leaves Film color 'Cuiyu' 'Plus cited number 1' P. laetevirens 'Cuiyu' P.quinquefolia 'Jiayin 1' QN VG (b Leaf. mature leaf Petiole cyan Glycoside coloring process 'Cuiyu' 'Plus cited number 1' 'Jindai' P. laetevirens 'Cuiyu' P.quinquefolia 'Jiayin 1' P.henryana 'Jindai' (*) QN MG (b) Early germination 'Plus cited number 1' 'Cuiyu' 'Jindai' P.quinquefolia 'Jiayin 1' P. laetevirens 'Cuiyu' P.henryana 'Jindai' QN MG (b) Early flowering 'Plus cited number 1' Dijin 'Jindai' P.quinquefolia 'Jiayin 1' P.tricuspidata P.henryana 'Jindai' QN MG (b) Phenology.

Fruit turn color

'Plus cited number 1' Dijin P.quinquefolia 'Jiayin 1' P.tricuspidata (*) QN MG (b) Phenology.

Leaf discoloration

(Only for autumn

Seasonal discoloration Species) 'Plus cited number 1' Dijin 'Jindai' P.quinquefolia 'Jiayin 1' P.tricuspidata P.henryana 'Jindai' (*) QN MG (b) Phenology.

Deciduous period

'Plus cited number 1' Dijin 'Jindai' P.quinquefolia 'Jiayin 1' P.tricuspidata P.henryana 'Jindai' A.2 Characteristic table illustration A.2.1 Characteristic table No. 7 The characteristics (branches. bolt wings) are illustrated in Figure A.1 no (Golden Brocade) Yes (Golden Brocade)

Figure A.1

A.2.2 Characteristic characteristics table No. 8 Feature (tendril. top shape when tender) The diagram is shown in Figure A.2 Inflated into a bead shape (ground brocade) and curled into a hook shape ('Plus Yin No. 1')

Figure A.2

A.2.3 Characteristic table No. 17 Feature (leaf. shape of central leaflet) The diagram is shown in Figure A.3 Oval broad oval obovate

Figure A.3

A.2.4 Characteristic table No. 18 Feature (leaf. shape of base of central leaflet) is shown in Figure A.4 Tapered wedge

Figure A.4

A.2.5 Characteristic characteristics table No. 19 Feature (leaf. edge type) diagram is shown in Figure A.5 Corrugated blunt serrated heavy serrated

Figure A.5

Appendix B

(Informative appendix) Technical Questionnaire Number (not required for applicants) 1. Variety name for registration (please specify Chinese name and scientific name) 2. Applicant Information Applicant. Co-applicant.

address.

Zip code. Phone. Fax. E-mail.

3. Origin of the variety Breed finder. Discovery date. Breeder. Breeding time.

Cross breeding. (female) (female) × (male) (parent) Live breeding. (female) (female) Other breeding routes.

Summary of the breeding process.

4. Main characteristics (The numbers in the parentheses in the first column are the trait characteristic numbers in Appendix A1. Please mark '' in [] after the corresponding characteristic code) 4.1 (1) New shoot. central color 1. green [] 2. green, pink [] 3. wine red [] 4.2 (2) New shoot. whether there is edge 1. No [] 2. Yes [] 4.3 (3) New tip. cross section shape 1. Round or oblate [] 2. Nearly square [] 4.4 (5) Branches. Whether the pores protrude 1. None [] 2. Yes [] 4.5 (8) Tendril. Type 1. Bead-shaped [] when young 2. Hooked top when young 4.6 (10) Leaf. Type 1. single leaf [] 2.3 leaflet compound leaf [] 3.5 leaflet compound leaf 4. Two type leaves [] 4.7 (15) Leaf. surface bubbly bulge 1. None or weak [] 2. Medium [] 3. Strong [] 4.8 (21) Leaf. Color of mature leaves 1. Green [] 2. Light green [] 3. Yellow [] 4.9 (23) Leaf. secondary color distribution of mature leaves 1. Leaf veins [] 2. Others [] 4.10 (27) Leaf. Discoloration at the end of the growing season 1. No [] 2. Yes [] 4.11 (29) Leaf. Petiole anthocyanin coloring 1. weak [] 2. medium [] 3. strong [] 5. Comparison information of similar varieties Variety names similar to this variety.

Typical differences from similar varieties.

6. Summary of variety characteristics (detailed description in accordance with the contents of the trait characteristics table in Appendix A1) 7. Additional information (ability to distinguish traits of varieties, etc.)

7.1 Stress resistance and adaptability (drought resistance, cold resistance, waterlogging resistance, salt and alkali resistance, to pests and diseases)

7.2 Reproduction points.

7.3 Main points of cultivation management.

7.4 Other information.

references

1.TG/1 General Introduction to the Examination of Distinctness, Uniformity and Stability and the Development of Harmonized Descriptions of New Varieties of Plants 2.TGP/5 Experience and Cooperation in DUS Testing 3.TGP/6 Arrangements for DUS Testing 4.TGP/7 Development of Test Guidelines 5.TGP/8 Use of Statistical Procedures in Distinctness, Uniformity and Stability Testing 6.TGP/9 Examining Distinctness 7.TGP/10 Examinng Uniformity