HJ 628-2011Regulation for the collection or genetic resources (English PDF)

Foreword

To implement the "People's Republic of China Environmental Protection Law", "People's Republic of China Wild Animal Protection Law", "medium Wild Plant Protection Regulations of the PRC "," Nature Reserve Regulations "People's Republic of China, etc. Regulate Health Collecting genetic resources, to strengthen the protection of genetic resources, promotion of research and the use of genetic resources and formulate this standard.

This standard specifies the procedures, technical specifications and precautions wildlife genetic resources within our collection.

This standard is the first release.

This standard is developed by the Ministry of Environmental Protection Science, Technology organization.

This standard is mainly drafted by. Environmental Protection Department of the Nanjing Institute of Environmental Sciences.

This standard MEP September 9, 2011 for approval.

This standard since January 1, 2012 implementation.

The standard explanation by the Ministry of Environmental Protection.

Genetic resources collection Technical Specification

1 Scope

This standard specifies the procedures, technical specifications and precautions wildlife genetic resources collected.

This standard applies to the territory of People's Republic of China wildlife genetic resources (animals, plants, fungi, large, etc.) collection.

2 Normative references

This standard references the following documents or the terms.

"National key protected wild animal list" (the letter [1988] No. 144) "National key protected wild plants (the first batch)" (the letter [1999] No. 92)

3 Terms and Definitions

The following terms and definitions apply to this standard.

4 Basic Principles

4.1 The principle of legality Collecting genetic resources shall not violate the provisions of relevant state laws and regulations, shall be collected before the relevant provisions of the State Relevant authorities or local application, to be implemented after the approval of the acquisition.

5 Procedures

Acquisition of basic biological genetic resources program comprising. gathering before preparation, implementation of collection, processing and storage of samples, Records.

6 Prepare, collected before

6.1 Acquisition national key protected wild animals, wild plant genetic resources (see "national key protected wild animal name Record "and" national key protected wild plants (first) "), shall be in accordance with the provisions of relevant laws and regulations to phase Off the competent authorities to apply, to be implemented after the approval of the acquisition. Need to collect genetic resources in nature reserves, shall Protected area management agencies to apply, to be implemented after the approval of the acquisition.

We should fully study its morphology, physiology and distribution of 6.2 before the acquisition of genetic resources and protection of endangered species, to avoid Collection activities cause irreparable damage.

6.3 Acquisition should be developed before a complete acquisition program, understand the distribution of regional wildlife, according to the local natural conditions made Given collection roadmap avoid collection activities aimless destruction of natural habitats.

6.4 collection route should be from the edge of the distribution of biological populations (populations) to the heart of the way forward, the priority acquisition Population (population) individual marginal distribution. In protected areas such as collection, shall order the experimental zone, buffer zone travel.

7 Acquisition officers

Respond collection personnel specialized training, to master the collection target species taxonomy, morphology and knowledge students Living habits, habitat and capture skills.

8 Collection of tools and materials

8.1 capture tools include. capturing network, homemade traps and tranquilizer gun (needle), etc., should be selected according to the collected objects.

8.2 processing equipment include. scissors (medical or scientific level), scalpel, tweezers, capped plastic tubes (or centrifugation Tube), capped jars, dropper, sealed plastic bags, tin foil cartridge, latex gloves, hand centrifuge. Equipment in advance Washed and disinfected to avoid contamination of tissue and infectious diseases.

8.3 recording tools include. marker, label paper, pencils and the like.

8.4 Materials handling and storage comprising. distilled water, liquid nitrogen, dry ice, 90% to 100% ethanol, buffer solution of DNA, anticoagulant Wait.

9 Collection methods and objects

10 Number, collected

We should try to collect a small amount of sample, usually 50 µl of blood or tissue to 200 mg. If the research or development work required to be appropriate to increase the collection amount, but not for the normal growth of the organism, affecting reproduction and activities. Should be strictly controlled weight Collection points and the amount of protection of endangered species.

Handling and storage of samples collected 11

11.1 Acquisition should wear sterile latex gloves, use forceps samples taken after sample collection with distilled water.

11.2 animal tissue into a clean plastic tube or jar, sealed and stored in liquid nitrogen or dry ice can also be dropped 90% to 100% ethanol or DNA buffer soaking the tissue preservation.

11.3 blood sample should be thoroughly mixed with anticoagulants, then wait for it to naturally settle or centrifuge from the use of the hand Heart, blood cells and plasma separation, discard the plasma, blood cells stored in liquid nitrogen or dry ice.

11.4 plant tissue in a plastic bag, 1.10 silica gel was added, followed by extrusion which air bag after closure Put tinfoil box stored.

11.5 samples taken at least two points to be stored in order to avoid risks. Samples should be sent to a laboratory experiment or within 1 week Long-term preservation.

12 Animals are treated

For the complete collection of animal genetic resources can not be abandoned, it should disinfect the wound and stopped the bleeding, wait for it to complete After waking up in situ release. Animals must not be taken outside the original habitat release.

13 Code of Ethics

When live animals are processed, the operation should be fast, be careful, try to alleviate their suffering.

14 Security measures

To make full safety precautions when collecting wild animal genetic resources. Capture and processing of wild beasts, snakes and other dangerous

Note that when the risk of animal safety, avoid for its injuries. For potential foci of animals such as rodents, birds, etc., should pay attention to anti Phytophthora protection, immunization injections if necessary.

15 Records

15.1 samples of genetic resources collected to timely numbered tag and do detailed records in order to provide the relevant competent ministries Doors and protected areas for the record.

Basic Contents 15.2 records shall include at least.

(1) Acquisition of basic human information (name, ID number, affiliation, address, contact information);

(2) approval authorities and approval document number;

(3) The purpose of the acquisition (the stated purpose of the use of genetic resources collection);

(4) Acquisition time;

(5) sample number;

(6) Location information collection (location, type of vegetation, habitat status, latitude and longitude coordinates, altitude, temperature , Humidity, photographs, etc.);

(7) Basic information Genetic Resources (Chinese name, scientific name, taxonomic status, collecting forms, collecting quantity) acquisition.

16 Repeat collection

In general, the acquisition must be submitted again to repeat acquisition. But for the purposes of scientific research universities and research centers which may be mutually agreed and relevant departments signed the book a certain period of acquisition plans.