HJ 1257-2022Chemical substances environmental management - Terminology for testing of chemical substances (English PDF)

2.1 General terms

2.1.1

test substances

The single chemical substance or mixture being tested.

2.1.2

Poorly water-soluble substances Substances with a solubility in water of less than 0.1 g/L at 20 °C.

2.1.3

Substances of unknown or variable composition, complex reaction products or biological materials; UVCB Substances, complex reaction products, or biological materials of unknown or variable composition.

2.1.4

difficult test chemicals Chemical substances in testing that are difficult to test by conventional standardized methods. It is mainly manifested that the conventional processing methods are not applicable, etc., which can be passed through Valid test results are obtained by partially modifying or supplementing standard test methods.

2.1.5

test organisms

Test animals, plants, microorganisms, etc. in the system. Also called test organism.

2.1.6

test solution

The mixture formed by a medium such as water or culture medium in which the test substance is dissolved or suspended and the test substance.

2.1.7

parent animals

In a breeding trial, the parent used for breeding at the start of the test is tested.

2.1.8

offspring

In breeding experiments, larvae produced by the parent.

2.1.9

loading rate

When preparing the test solution of the test substance that is insoluble in water, the ratio of the test substance to the test medium is usually expressed in mg/L. for unstable Test object, the bearing rate is expressed by the test object itself. In fish-related tests, it also refers to the wet weight of fish per unit volume of water.

2.1.10

octanol-water partition coefficient; Kow; Pow the concentration ratio of the test substance when it reaches equilibrium in a two-phase medium of n-octanol and water.

2.1.11

adsorption coefficient; Koc the ratio of the concentration of the test substance when it reaches equilibrium in an organic medium (soil or sediment) and an aqueous two-phase medium.

2.1.12

water-accommodated fraction; WAF the total dispersion of a multi-component test substance in water, including the dissolved, suspended and/or emulsified fractions.

2.1.13

water soluble fraction; WSF the water-loaded fraction is subjected to separation steps (eg, centrifugation, filtration) to remove insoluble suspended and emulsified fractions.

2.1.14

static test

During the test, the test mode of the test solution is not updated.

2.1.15

semi-static renewal test in the test, in order to ensure a certain concentration of the test substance, there is a plan to replace the test solution regularly.

2.1.16

flow-through test

A test in which a test organism is exposed to a continuous or intermittent flow of a test solution.

2.1.17

limit test

A test given an upper dose or concentration. Usually the test substance is expected to be less toxic or less soluble and no predictor was observed in pilot studies Limit test can be carried out when the period effect is not observed.

2.1.18

photoperiod Alternate cycles of light and dark conditions within 24 hours.

2.1.19

test endpoint

Compared with the control group, the measurable characteristic index that the test substance changes the test organism, the commonly used test endpoints are mortality, inhibition rate Wait.

2.1.20

mortality

The proportion of the number of test organisms with no signs of life activity to the total number of test organisms, usually expressed as a percentage.

2.1.21

biomass

Dry weight of living material present in a population under test, may also refer to number of leaves, leaf area, number of cells per unit volume or fluorescence Alternative indicators such as light value.

2.1.22

growth yield

The difference between the biomass at the end of the test and the start of the test.

2.1.23

Reproductive output

The total number of surviving offspring produced by the parent during the test period.

2.1.24

hatchability

Percentage of hatched eggs (eggs) in a breeding test such as birds or fish.

2.1.25

embryo viability

Percentage of hatched eggs that have begun embryonic development after fertilization in reproductive testing.

2.1.26

behavioral symptoms

The abnormal behavior of the test organism after exposure to the test substance is one of the indicators of harmful effects.

2.1.27

coefficient of variation coefficient of variation; CV the ratio of the standard deviation to the mean, dimensionless, usually expressed as a percentage.

2.1.28

dosedose

The amount of the test substance that enters the test organism and interacts with metabolic processes or important receptors. When testing, usually refers to the administration of the test substance amount or input.

2.1.29

dose-response (effect) relationship Quantitative relationship between dose and biological response (effect) in a test.

2.1.30

no lethal concentration; LC0 the highest concentration of the test substance that did not cause the death of any test organism during a given test period.

2.1.31

total lethal concentration; LC100 the lowest concentration of the test substance that causes 100% of the test organisms to die during a given test period.

2.1.32

median effect concentration; EC50 the concentration of the test substance that causes an observed effect in 50% of the test organisms during a given test period.

2.1.33

effect concentration; ECx the concentration of the test substance that results in an observed effect in x% of the test organisms compared to the control group during a given test period.

2.1.34

median lethal dose (concentration); LD50; LC50;

The dose or concentration of the test substance that causes the death of 50% of the test organisms during a given test period.

2.1.35

median lethal dietary dose; LDD50 at the end of the oral acute/chronic toxicity test, the ingested dose that caused the death of 50% of the tested organisms.

2.1.36

x% decay time disappearance time x; DTx the time required for the concentration of the test substance to decrease to x% of the initial concentration. If the transformation process of the test substance follows the first-order kinetic equation, the 50% decay time (DT50) is the same as the half-life (t1/2).

2.1.37

Half-life; t1/2 the time required for the concentration of the test substance to decrease to 50% is generally used to describe the first-order reaction rate. Half-life and degradation rate constant (k) the relation is t1/2 = ln 2/k.

2.1.38

lowest observed effect concentration; LOEC the lowest level that produced a statistically significant (p