GBZ/T 317.2-2018Determination of cadmium in blood -- Part 2: Inductively coupled plasma mass spectrometry method (English PDF)

Foreword

This standard is formulated in accordance with the Law of the People's Republic GBZ/T 317 "Determination of Cadmium in Blood" is divided into two parts.

-- Part 1. Graphite furnace atomic absorption spectrometry.

-- Part 2. Inductively coupled plasma mass spectrometry.

This part is the second part of GBZ/T 317.

This part is drafted in accordance with the rules given in GB/T 1.1-2009.

This section is mainly drafted by. China Center for Disease Control and Prevention, Occupational Health and Poison Control Institute, Guangdong Provincial Occupational Disease Prevention and Treatment Institute, Jiangsu Provincial Center for Disease Control and Prevention, Shandong Institute of Occupational Health and Occupational Diseases.

1 Scope

This part of GBZ/T 317 specifies inductively coupled plasma mass spectrometry for the determination of cadmium in blood.

This section applies to the determination of cadmium and its compounds in the blood of occupational contacts.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document.

For undated references, the latest edition (including all amendments) applies to this document.

GBZ/T 295 General rules for occupational health biological monitoring

3 Principle

Blood samples (hereinafter referred to as blood samples) were directly diluted with 0.5% nitric acid/0.01% Triton X-100 mixture to give (Rh, 10.0 µg/L) as an internal standard, the concentration of cadmium in the diluent was measured by inductively coupled plasma mass spectrometry.

4 Instruments

4.1 Blood collection supplies. disposable blood collection needle, sterile cotton swab, lithium heparin (sodium) vacuum blood collection tube, etc.

4.2 Volumetric flask. 5 mL.

4.3 Polyethylene centrifuge tube. 15 mL.

4.4 Vortex mixer.

4.5 Micropipette. The range is from 100 µL to 1000 µL.

4.6 Inductively Coupled Plasma Mass Spectrometer. equipped with an autosampler.

Instrument operation reference conditions.

-- cooling gas flow rate. 12.5 L/min;

-- Auxiliary gas flow rate. 0.70 L/min;

- atomization gas flow rate. 0.88 L/min;

- peristaltic pump speed. 30 r/min;

-- Nebulizer temperature. 3 °C.

Note. The above conditions are provided for the convenience of standard users. If the conditions are changed, the same effect should be achieved.

5 Reagent

5.1 Deionized water.

5.2 Nitric acid, excellent grade pure, rho20=1.42 g/mL; nitric acid solution, 0.2% (volume fraction).

5.3 0.5% nitric acid/0.01% Triton X-100 mixed solution.

5.4 Heparin anticoagulated bovine blood, stored at -20 °C, shake to room temperature when used. Heparin anticoagulated sheep blood can also be used. The background cadmium content should be lower than 0.2 µg/L.

5.5 Standard solution. Adopt nationally recognized standard solution of cadmium and strontium.

6 Sample collection, transportation and storage

6.1 Environmental conditions. The blood collection site should be clean and tidy, and there is no source of cadmium pollution.

6.2 Personnel requirements. After rigorous training and familiarity with the blood collection process, wear powder-free latex gloves.

6.3 Sample collection. 0.2% nitric acid solution, deionized water, and clean skin of the blood collection site, the blood sample should be collected more than 2 mL, and Shake immediately to prevent clot formation in the blood. Collect duplicate samples as necessary and one copy as a backup sample.

6.4 Sample blank. randomly take 2 parts of the blood collection container with the same batch number as the sample collection, and use the same batch of blood collection needle to extract 2.0 mL water into the blood collection tube.

Medium, as a sample blank.

6.5 Sample transportation. Store the collected samples and sample blanks in a clean container for refrigerated transport.

6.6 Sample storage. The sample can be stored for half a year at -20 °C.

7 Analysis steps

8 Calculation

Calculate the concentration of cadmium in the blood sample according to formula (1).

C = F × C0 (1)

In the formula.

C - the concentration of cadmium in the blood, in micrograms per liter (µg/L);

F - sample dilution factor (10 in this method);

C0 - The concentration of cadmium in a blood sample calculated from a standard curve or a regression equation in micrograms per liter (µg/L).

9 Description

9.1 The detection limit of this method is 0.08 µg/L, the lower limit of quantification is 0.30 µg/L; the measurement range is 0.30 µg/L to 1000.00 µg/L (in Blood sample 10 times diluted). The relative standard deviation ranged from 2.60% to 8.70% (n=6).

9.2 Single-use items such as vacuum blood collection tubes, polyethylene centrifuge tubes and micro-pump tips should also be used after sampling.

The sampling amount of each batch number shall not be less than 10. Volumetric flasks and other supplies need to be soaked overnight in 1..1 nitric acid solution, followed by tap water, deionized the water is cleaned, and the background test is carried out by adding 0.5% nitric acid solution as the liquid to be tested, and the test result should be less than the detection limit.

9.3 The EDTA anticoagulation tube cannot be used for blood collection tubes.

9.4 The quality control of the testing process shall be carried out in accordance with the requirements of GBZ/T 295.