GBZ/T 314-2018Determination of nickel in blood -- Graphite furnace atomic absorption spectrometry method (English PDF)

Foreword

This standard is formulated in accordance with the Law of the People's Republic this standard was drafted in accordance with the rules given in GB/T 1.1-2009.

This standard replaces WS/T 45-1996 "Method for the determination of nickel in blood by graphite furnace atomic absorption spectrometry".

The main technical changes compared to WS/T 45-1996 are as follows.

-- Improved sample handling method with 0.1% Triton X-100 solution as diluent.

This standard was drafted. Shenzhen Occupational Disease Prevention and Treatment Institute, Shenzhen Baoan District Center for Disease Control and Prevention, Yantian District, Shenzhen, disease prevention Control Center, Shenzhen Luohu District Center for Disease Control and Prevention.

1 Scope

This standard specifies graphite furnace atomic absorption spectrometry for the determination of nickel in blood.

This standard applies to the determination of nickel in the blood of occupational contacts.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document.

For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 Analytical laboratory water specifications and test methods General rules for biological monitoring of GBZ/T 295 occupational population

3 Principle

Blood samples were diluted with 0.1% Triton X-100 solution and measured by graphite furnace atomic absorption spectrometry at 232.0 nm.

4 Instruments

4.1 Atomic absorption spectrophotometer with graphite furnace, Zeeman or other background correction device and nickel hollow cathode lamp.

4.2 Volumetric flask, 50 mL, 100 mL.

4.3 Analyze the balance with a sensation of 0.1 mg.

4.4 Pipettes, 50 L, 100 L,.200 L, 1000 L.

4.5 covered plastic centrifuge tube, 2 mL.

4.6 vortex oscillator.

5 Reagent

5.1 Experimental water. Performed in accordance with GB/T 6682 standard.

5.2 Nitric acid. rho20=1.42 g/mL, excellent grade pure.

5.3 Heparin sodium blood collection tube. excellent grade pure.

5.4 Nitric acid solution. 5 mL of nitric acid was added to 95 mL of water.

5.5 Heparin sodium solution. Weigh 0.5 g of sodium heparin, dissolve in water, add water to 100 mL.

5.6 Triton X-100. Analytical grade.

5.7 Diluent. Take 0.1 mL of Triton X-100, dissolve in an appropriate amount of water, and dilute to 100 mL.

5.8 Nickel Standard Solution. Prepared with a nationally recognized nickel standard solution.

6 Sample collection, transportation and storage

2 mL of blood samples exposed to nickel workers were collected with heparin sodium anticoagulated blood vessels and transported at room temperature or refrigerated.

The samples were stored at 4 ° C and stored for up to 7 days. If it is not refrigerated, store it at room temperature for up to 3 days.

7 Analysis steps

8 Calculation

Calculate the concentration of nickel in the blood according to formula (1).

X = 10c ... (1)

In the formula.

X - the concentration of nickel in the blood, in micrograms per liter (g/L);

10 -- blood sample dilution factor;

c -- The concentration of nickel in the blood sample after treatment (minus the sample blank) in micrograms per liter (g/L).

9 Description

9.1 The detection limit of this method is 1.13 g/L; the minimum detection concentration is 11.3 g/L (10 times dilution); the lower limit of quantification is 3.76 g/L;

The minimum quantitative concentration is 37.6 g/L (10 times dilution); the linear range of the working curve is 1.13 g/L~160 g/L;

The quasi-deviation. RSD=1.2%~4.3%; the recovery rate of blood samples was 98.5 %~108.0%.

9.2 Equipment cleaning. Glass and plastic utensils are soaked in 10% nitric acid solution for about 12 h, rinse off. Dust away after drying.

9.3 Sampling requirements. For workers exposed to soluble nickel salts, post-class blood should be collected, which represents the contact situation for one working day. Before sampling, Workers should take off the contact site, take off their overalls, wash hands, face and sampling area, and then sample in a clean, non-polluting place. In turn Thoroughly scrub the sampling site with 0.5% nitric acid and deionized water, then sterilize and take blood to prevent external contamination. After blood collection, shake gently to make blood Mix the liquid thoroughly with the anticoagulant, but avoid strong shaking. Sample collectors must be proficient in the knowledge of sample collection, transport and preservation.

technology.

9.4 When the blood nickel concentration is 457 g/L, 1000 g/mL of sodium, potassium, calcium, magnesium, copper, manganese, chromium, lead, zinc, arsenic, cadmium, zirconium, 500 g/mL of iron, cobalt, 100 g/mL of lithium, lanthanum, cerium, lanthanum, cerium, lanthanum, vanadium, molybdenum and tungsten did not interfere with the measurement.

9.5 If the blood nickel concentration is outside the measurement range, the dilution factor may be increased, but the normal human blood in the working curve should also be the same dilution factor.

9.6 The quality assurance of the testing process shall be carried out in accordance with the requirements of GBZ/T 295.