GB/T 9695.6-2008Meat products -- Determination of artificial colour ponceau 4R (English PDF)

Foreword

GB/T 9695 consists of the following components.

--- GB/T 9695.1 "Meat and meat products - Determination of free fat content";

--- GB/T 9695.2 "Meat and meat products - Determination of fatty acid";

--- GB/T 9695.3 "Meat and meat products - Determination of iron content";

--- GB/T 9695.4 "Meat and meat products - Determination of total phosphorus content";

--- GB/T 9695.5 "Meat and meat pH measurement";

--- GB/T 9695.6 "meat Carmine colorant determination";

--- GB/T 9695.7 "Meat and meat products - Determination of total fat content";

--- GB/T 9695.8 "Meat and meat products - Determination of chloride content";

--- GB/T 9695.9 "Meat and meat products polyphosphate determination";

--- GB/T 9695.10 "meat and meat products BHC and DDT residues determination";

--- GB/T 9695.11 "Meat and meat products - Determination of nitrogen content";

--- GB/T 9695.13 "Meat and meat products - Determination of calcium content";

--- GB/T 9695.14 "meat starch content determination";

--- GB/T 9695.15 "Meat and meat products - Determination of moisture content";

--- GB/T 9695.17 "Meat and meat products - Determination of glucono-lactone content of -delta-";

--- GB/T 9695.18 "Meat and meat products - Determination of ash";

--- GB/T 9695.19 "Meat and meat sampling method";

--- GB/T 9695.20 "Meat and meat products - Determination of zinc";

--- GB/T 9695.21 "Meat and meat products - Determination of magnesium content";

--- GB/T 9695.22 "Meat and meat products - Determination of copper content";

--- GB/T 9695.23 "Meat and meat products L (-) - hydroxyproline determination";

--- GB/T 9695.24 "Meat and meat cholesterol content determination";

--- GB/T 9695.25 "Meat and meat products - Determination of vitamin PP";

--- GB/T 9695.26 "Meat and meat products - Determination of vitamin A content";

--- GB/T 9695.27 "Meat and meat products - Determination of vitamin B1";

--- GB/T 9695.28 "Meat and meat products - Determination of vitamin B2";

--- GB/T 9695.29 "Meat vitamin C content determination";

--- GB/T 9695.30 "Meat and meat products - Determination of vitamin E content";

--- GB/T 9695.31 "Meat total sugar content determination." this section GB/T 9695 Part 6 of Zi.

This Part replaces GB/T 9695.6-1988 "meat Carmine colorant determination." Section compared with the GB/T 9695.6-1988 The main changes are as follows.

--- According to GB/T 1.1-2000 "Standardization Guide Part 1. Standard structure and writing rules" and GB/T 20001.4-2001 "Rules for drafting standards - Part 4. Chemical analysis method" has been restructured and text modify;

--- Increasing the normative references;

--- Increasing the detection limit;

--- By HPLC method as the first, as the second colorimetric determination Meat carmine colorant; reagents, instruments Equipment, analytical steps, make corresponding changes to the results of the calculation;

--- "Purification" step with ethanol + + aqueous ammonia instead of ammonia solution as desorption solution;

--- With Chapter 10, "precision" and its contents instead of GB/T 9695.6-1988, Chapter 9, "allows poor" and its contents;

--- Added "Test Report" chapter;

--- Increase the informative Appendix A.

Appendix A of this section is an informative annex.

This part of the National Technical Committee of Standardization Technical Committee eggs and meat products food industry and focal points.

This section drafted by. China Chamber of Commerce Business Standard Centre, the National Food Quality Supervision and Inspection Center Processing (Guangzhou), Guangzhou Product quality supervision and inspection.

1 Scope

GB/T 9695 provisions of this part of the determination of meat products carmine colorant.

The first law of this section apply to meat products Carmine measured colorant, the second law applies to meat products containing only carmine colorant Determination of the Carmine.

The detection limit of this method. HPLC. 0.05mg/kg; colorimetry. 0.4mg/kg.

2 Normative references

The following documents contain provisions which, through reference to GB/T 9695 to the present, constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest version of the referenced files. For undated reference documents, the latest versions apply to this section.

Laboratory use specifications and test methods (. 1987 neq ISO 3696) GB/T 6682-1992 Analysis the first method HPLC Principle 3 Carmine in the sample by sample degreasing, alkaline solution extraction, protein precipitation, polyamide powder adsorption, ethanol + ammonia + water desorption After an aqueous solution, was measured by HPLC after filtration. Based on retention time qualitative, external standard.

4 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade.

4.1 Water. Water line with two GB/T 6682-1992 requirements.

4.2 Methanol. chromatographically pure.

4.3 formic acid.

4.4 petroleum ether. boiling range 30 °C ~ 60 °C.

4.5 ethanol.

4.6 sodium tungstate.

4.7 citric acid.

4.8 ammonium acetate.

4.9 sea sand. chemically pure. Be boiled with hydrochloric acid solution (1 + 10) 15min, washed with water (4.1) washed until neutral, and then 50g/L sodium hydroxide solution Liquid boiled 15min, washed with water (4.1) washed until neutral, dried at 105 °C, stored in a stoppered bottle.

Sulfuric acid solution 4.10 [1 + 9 (volume ratio)]. the amount of 10mL of concentrated sulfuric acid, while stirring slowly added to 90mL of water.

4.11 sodium tungstate (Ba = 100g/L). Weigh 10g sodium tungstate dissolved in water and diluted to 100mL.

4.12 ammonium acetate (Ba = 0.02mol/L). Weigh 1.54g ammonium acetate, dissolved in water and diluted to 1000mL. Filtered through 0.45µm Membrane filtration.

4.13 citric acid solution (Ba = 200g/L). Weigh 20g of citric acid (C6H8O7 · H2O), dissolved in water and diluted to 100mL.

Methanol, formic acid + 4.14 [3 + 2 (volume ratio)]. amount of methanol 60mL, 40mL formic acid, and mix.

4.15 ammonia + water + ethanol [7 + 2 + 1 (by volume)]. amount of ethanol 70mL, 20mL ammonia, 10mL water, and mix.