GB/T 9695.28-2008Meat and meat products -- Determination of vitamin B2 content (English PDF)

Foreword

GB/T 9695 consists of the following components.

--- GB/T 9695.1 "Meat and meat products - Determination of free fat";

--- GB/T 9695.2 "Meat and meat products - Determination of fatty acid";

--- GB/T 9695.3 "Meat and meat products - Determination of iron content";

--- GB/T 9695.4 "Meat and meat products - Determination of total phosphorus content";

--- GB/T 9695.5 "Meat and meat pH measurement";

--- GB/T 9695.6 "meat Carmine colorant determination";

--- GB/T 9695.7 "Meat and meat products - Determination of total fat content";

--- GB/T 9695.8 "Meat and meat products - Determination of chloride content";

--- GB/T 9695.9 "Meat and meat products polyphosphate determination";

--- GB/T 9695.10 "meat and meat products BHC and DDT residues determination";

--- GB/T 9695.11 "Meat and meat products - Determination of nitrogen content";

--- GB/T 9695.13 "Meat and meat products - Determination of calcium content";

--- GB/T 9695.14 "meat starch content determination";

--- GB/T 9695.15 "Meat and meat products - Determination of moisture content";

--- GB/T 9695.17 "Meat and meat products - Determination of glucono-lactone -delta- content";

--- GB/T 9695.18 "Meat and meat products - Determination of ash";

--- GB/T 9695.19 "Meat and meat sampling method";

--- GB/T 9695.20 "Meat and meat products - Determination of zinc";

--- GB/T 9695.21 "Meat and meat products - Determination of magnesium content";

--- GB/T 9695.22 "Meat and meat products - Determination of copper content";

--- GB/T 9695.23 "Meat and meat products - Determination of hydroxyproline content";

--- GB/T 9695.24 "Meat and meat cholesterol content determination";

--- GB/T 9695.25 "Meat and meat products - Determination of vitamin PP";

--- GB/T 9695.26 "Meat and meat products - Determination of vitamin A content";

--- GB/T 9695.27 "Meat and meat products - Determination of vitamin B1";

--- GB/T 9695.28 "Meat and meat products - Determination of vitamin B2";

--- GB/T 9695.29 "Meat vitamin C content determination";

--- GB/T 9695.30 "Meat and meat products - Determination of vitamin E content";

--- GB/T 9695.31 "Meat total sugar content determination." this section GB/T Part of 289,695.

This Part replaces GB/T 9695.28-1991 "Meat and meat products - Determination of vitamin B2." this section compared with the GB/T 9695.28-1991 The main changes are as follows.

--- According to GB/T 1.1-2000 "Standardization Guide Part 1. Standard structure and writing rules" and GB/T 20001.4-2001 "Rules for drafting standards - Part 4. Chemical analysis methods" for the restructuring and editorial changes;

--- By HPLC method as the first, as the second colorimetric determination of meat and meat products in the content of vitamin B2, reagents, Equipment, analysis step, make corresponding changes to the results of the calculation;

--- The "sample" chapter is divided into "sampling" and "Sample Preparation" two chapters;

--- With "precision 10" and its contents instead of "9 to allow poor" and its contents;

--- Added "Test Report" chapter.

Appendix A of this section is an informative annex.

This part of the National Standardization Technical Committee of poultry meat products and centralized.

This section drafted by. China Chamber of Commerce Business Standard Centre, the National Food Quality Supervision and Inspection Center Processing (Guangzhou), Guangzhou Product quality supervision and inspection.

1 Scope

GB/T 9695 provisions of this part of the determination of meat and meat products of vitamin B2 content.

This section applies to meat Determination of vitamin B2 and meat products.

2 Normative references

The following documents contain provisions which, through reference to GB/T 9695 to the present, constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section.

GB/T 6682-2008 analytical laboratory use specifications and test methods (ISO 3696. 1987, MOD) the first method HPLC Principle 3 Samples with dilute acid after 121 °C ~ 123 °C autoclave processed to extract vitamin B2 (riboflavin), filtered, measured by high performance liquid chromatography set. Retention time qualitative, external standard.

4 Reagents

Unless otherwise indicated, the reagents used were of analytical grade.

4.1 Water. Water line with a GB/T 6682-2008 requirements.

4.2 glacial acetic acid.

4.3 Methanol. chromatography or by redistilled.

4.4 hydrochloric acid solution (Ba = 0.1mol/L). draw 2.1mL hydrochloric acid (rho20~1.19g/mL), diluted with water to 250mL, and mix.

4.5 sodium hydroxide solution (Ba = 1mol/L). Weigh 10g of sodium hydroxide, dissolved in water and dilute to 250mL.

4.6 acetic acid solution (Ba = 0.02mol/L). lessons 1.14mL glacial acetic acid (4.2), diluted with water to 1L.

4.7 sodium acetate solution [Ba = 0.05mol/L (pH 4.5)]. Weigh 3.4g of sodium acetate trihydrate, dissolved in water and diluted with water to 500mL, glacial acetic acid (4.2) adjusted to pH 4.5.

Precision pH test paper 4.8. 3.8 to 5.4 range.

4.9 Vitamin B2 standard solution.

4.9.1 Standard stock solution (Ba = 100µg/mL). Weigh 50.0mg vitamin B2 standards, dissolved in acetic acid solution (4.6), and set the volume Brown to 500mL volumetric flask (can be dissolved by heating on a water bath, then cooled to volume).

4.9.2 standard working solution (Ba = 0.05µg/mL). draw stock standard solution (4.9.1) 1.00mL in 100mL brown volumetric flask Acetic acid solution (4.6) to volume, and mix. Draw solution 5.00mL in 100mL brown volumetric flask with water to volume, and mix. When you use with system.

5 Apparatus

Conventional laboratory equipment and the following instruments.

5.1 Mechanical Equipment. For homogenization of the sample. Including high-speed rotation of the cutter, or no more than 4mm diameter of the orifice of the meat grinder.