GB/T 9695.24-2008Meat and meat products -- Determination of cholesterol (English PDF)

Foreword

GB/T 9695 consists of the following components.

--- GB/T 9695.1 "Meat and meat products - Determination of free fat";

--- GB/T 9695.2 "Meat and meat products - Determination of fatty acid";

--- GB/T 9695.3 "Meat and meat products - Determination of iron content";

--- GB/T 9695.4 "Meat and meat products - Determination of total phosphorus content";

--- GB/T 9695.5 "Meat and meat pH measurement";

--- GB/T 9695.6 "meat Carmine colorant determination";

--- GB/T 9695.7 "Meat and meat products - Determination of total fat content";

--- GB/T 9695.8 "Meat and meat products - Determination of chloride content";

--- GB/T 9695.9 "Meat and meat products polyphosphate determination";

--- GB/T 9695.10 "meat and meat products BHC and DDT residues determination";

--- GB/T 9695.11 "Meat and meat products - Determination of nitrogen content";

--- GB/T 9695.13 "Meat and meat products - Determination of calcium content";

--- GB/T 9695.14 "meat starch content determination";

--- GB/T 9695.15 "Meat and meat products - Determination of moisture content";

--- GB/T 9695.17 "Meat and meat products - Determination of glucono-lactone -delta- content";

--- GB/T 9695.18 "Meat and meat products - Determination of ash";

--- GB/T 9695.19 "Meat and meat sampling method";

--- GB/T 9695.20 "Meat and meat products - Determination of zinc";

--- GB/T 9695.21 "Meat and meat products - Determination of magnesium content";

--- GB/T 9695.22 "Meat and meat products - Determination of copper content";

--- GB/T 9695.23 "Meat and meat products - Determination of hydroxyproline content";

--- GB/T 9695.24 "Meat and meat cholesterol content determination";

--- GB/T 9695.25 "Meat and meat products - Determination of vitamin PP";

--- GB/T 9695.26 "Meat and meat products - Determination of vitamin A content";

--- GB/T 9695.27 "Meat and meat products - Determination of vitamin B1";

--- GB/T 9695.28 "Meat and meat products - Determination of vitamin B2";

--- GB/T 9695.29 "Meat vitamin C content determination";

--- GB/T 9695.30 "Meat and meat products - Determination of vitamin E content";

--- GB/T 9695.31 "Meat total sugar content determination." this section GB/T 9695 of Part 24.

This Part replaces GB/T 9695.24-1990 "Meat and meat products - Determination of cholesterol." this section compared with the GB/T 9695.24-1990 The main changes are as follows.

--- According to GB/T 1.1-2000 "Standardization Guide Part 1. Standard structure and writing rules" and GB/T 20001.4-2001 "Rules for drafting standards - Part 4. Chemical analysis method" has been restructured and text modify;

--- Canceled the step Soxhlet fat sample, the samples were directly saponified;

--- External standard method instead of the internal standard;

--- Added "Sample Preparation" chapter;

--- In the "Determination" chapter, instead of filling by capillary column for separation, increasing the "parallel trials" and "blank";

--- Formulas to do the appropriate changes;

--- With Chapter 10, "precision" and its contents instead of Chapter 9, "allows poor" and its contents;

--- Added "Test Report" chapter.

Appendix A of this section is an informative annex.

This part of the National Standardization Technical Committee of poultry meat products and centralized.

This section drafted by. China Chamber of Commerce Business Standard Centre, the National Food Quality Supervision and Inspection Center Processing (Guangzhou), Guangzhou Product Quality Supervision, Inspection, Zhejiang Institute of Quality and Technical Supervision and Inspection.

1 Scope

GB/T 9695 provisions of this part of the determination of meat and meat cholesterol content.

This section applies to the determination of cholesterol content of meat and meat products.

2 Normative references

The following documents contain provisions which, through reference to GB/T 9695 to the present, constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section.

GB/T 6682-2008 analytical laboratory use specifications and test methods (ISO 3696. 1987, MOD) Principle 3 After the meat and meat by saponification of lipids, cholesterol as unsaponifiables are extracted, measured by gas chromatography and quantified by external standard.

4 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade.

4.1 Water. Water line with two GB/T 6682-2008 requirements.

4.2 ether.

4.3 chloroform.

4.4 ethanol.

4.5 over anhydrous sodium sulfate.

4.6 potassium hydroxide solution (Ba = 1mol/L). Weigh 5.6g of potassium hydroxide, dissolved in water and diluted with water to 100mL.

4.7 Cholesterol Standard. Purity >=95%.

4.8 cholesterol standard stock solution (Ba = 1.0mg/mL). Weigh cholesterol standard (4.7) 0.1000g, set 100mL flask, Chloroform was dissolved and set the volume.

4.9 cholesterol standard working solution. When you use the cholesterol standard stock solution (4.8) was diluted with ethanol (4.4) to the desired concentration.

5 Apparatus

Conventional laboratory equipment and the following instruments.

5.1 Mechanical Equipment. For homogenization of the sample. Including high-speed rotation of the cutter, or no more than 4mm diameter of the orifice of the meat grinder.

5.2 Gas chromatograph. with capillary column and FID detector.

5.3 Electronic balance. weighing accurately to 0.1mg.

6 Sampling

Laboratory samples should be representative received and no damaged or changed during transport and during storage.

Sampling method, see GB/T 9695.19.

Take a representative sample 200g.