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GB/T 47208-2026Diagnostic techniques for fasciolosis (English PDF)

片形吸虫病诊断技术

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

February 27, 2026

Implementation date

September 1, 2026

Scope

GB/T 47208-2026 is the English-translated version of 片形吸虫病诊断技术.

GB/T 47208-2026 is the Chinese standard "Diagnostic techniques for fasciolosis". Its scope clause reads: This document describes the clinical diagnosis, etiological examination, polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA) for schistosomiasis.

Diagnostic methods such as ELISA. This document applies to the diagnosis, monitoring, epidemiological investigation, and quarantine of fascioliasis in cattle and sheep, and includes etiological examination and detection techniques.

Suitable for evaluating the effectiveness of prevention and control. Its clauses include terms and definitions; abbreviations.

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Document preview — GB/T 47208-2026

National Standard of the People's Republic of China

ICS: 11.220
Classification: B 41

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Preface
Introduction
1.Scope
2 Normative References
3.Terms and Definitions
4.Abbreviations
5.Clinical diagnosis
6.Sample Collection and Processing
7.Pathogen examination
8 PCR testing
9.Indirect ELISA 7.
10.Comprehensive Judgment
Appendix A (Standardized) Preparation of Reagents for Pathogen Examination
Appendix B (Informative) Typical Morphological Characteristics of Insect Eggs and Insects
Appendix C (Standard) PCR Solution Preparation
Appendix D (Informative) PCR primer locations, standard positive plasmids, and electrophoresis diagram
Appendix E (Normative) Reagent Preparation Methods for Indirect ELISA Methods
Appendix F (Informative) Preparation of ESP antigen from Fasciola hepatica
Appendix G (Informative) Example of sample loading for indirect ELISA test

1 Scope

This document describes the clinical diagnosis, etiological examination, polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA) for schistosomiasis.

Diagnostic methods such as ELISA.

This document applies to the diagnosis, monitoring, epidemiological investigation, and quarantine of fascioliasis in cattle and sheep, and includes etiological examination and detection techniques.

Suitable for evaluating the effectiveness of prevention and control.

2 Normative references

The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.

For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.

This document.

GB/T 6682 Specifications and test methods for water used in analytical laboratories

NY/T 541 Technical Specifications for Veterinary Diagnostic Sample Collection, Preservation and Transportation

3 Terms and Definitions

This document does not contain any terms or definitions that need to be defined.

4 Abbreviations

The following abbreviations apply to this document.

BSA. Bovine Serum Albumin

DMSO. Dimethyl sulfoxide

EB. Ethidium Bromide

EPG. Eggs Per Gram (feces)

ESP. Excretory-Secretory Product

IgG. Immunoglobulin G

OD. Optical Density

OVA. Ovalbumins

PBST. Phosphate buffer containing 0.5% Tween-20 […]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 22 pages — is available in the English PDF.

Referenced standards

Normative references

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