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GB/T 47203-2026Diagnostic techniques for orientobilharziasis (English PDF)

东毕吸虫病诊断技术

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

February 27, 2026

Implementation date

September 1, 2026

Scope

GB/T 47203-2026 is the English-translated version of 东毕吸虫病诊断技术.

GB/T 47203-2026 is the Chinese standard "Diagnostic techniques for orientobilharziasis". Its scope clause reads: This document describes the clinical diagnosis, sample collection, transportation and processing, pathogen morphological examination, and PCR detection methods for East Asian trematode disease.

Real-time PCR detection methods and indirect hemagglutination assays are among the methods used. This document applies to the diagnosis, detection, and epidemiological investigation of East Asian trematode disease. Its clauses include terms and definitions; abbreviations.

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Document preview — GB/T 47203-2026

National Standard of the People's Republic of China

ICS
11.220
Classification
B 41

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • Preface
  • Introduction
  • 1.Scope
  • 2 Normative References
  • 3.Terms and Definitions
  • 4.Abbreviations
  • 5.Clinical diagnosis
  • 6.Sample Collection, Transportation and Processing
  • 7.Pathogen morphological examination methods
  • 8.PCR detection methods
  • 9 Real-time PCR detection methods
  • 10.Indirect Hemagglutination Assay (IHA)
  • 11.Comprehensive Judgment
  • Appendix A (Informative) Pathological Changes in East Asian Paragonimiasis
  • Appendix B (Informative) Morphology of East Asian trematode eggs and bodies
  • Appendix C (Normative) Electrophoresis Reagent Preparation Method
  • Appendix D (Informative) Preparation of Positive Plasmids
  • Appendix E (Informative) Preparation of Negative and Positive Control Serums
  • Appendix F (Informative) PCR Amplification Product Sequences and Result Interpretation Figure
  • Appendix G (Informative) Real-time PCR Detection Quality Control Standards and Result Interpretation Figure
  • Appendix H (Normative) Preparation Method of Indirect Hemagglutination Test Reagent

1 Scope

This document describes the clinical diagnosis, sample collection, transportation and processing, pathogen morphological examination, and PCR detection methods for East Asian trematode disease.

Real-time PCR detection methods and indirect hemagglutination assays are among the methods used.

This document applies to the diagnosis, detection, and epidemiological investigation of East Asian trematode disease.

2 Normative references

The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.

For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.

This document.

NY/T 541 Technical Specifications for Veterinary Diagnostic Sample Collection, Preservation and Transportation

3 Terms and Definitions

The following terms and definitions apply to this document.

3.1 Insect eggs

The eggs produced after the female and male insects mate.

3.2 cercaria

The late-stage larvae that develop from sporocysts and daughter sporocysts within an intermediate host.

3.3 soluble antigen

Antigens released after the parasite is lysed and dissolved in physiological saline.

4 Abbreviations

The following abbreviations apply to this document.

Ct value. cyclethreshold

TAE. Tris-aceticacid-EDTA electrophoresis buffer […]

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 23 pages — is available in the English PDF.

Referenced standards

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