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GB/T 47130-2026Diagnostic techniques for bovine coronavirus infection (English PDF)

牛冠状病毒感染诊断技术

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

January 28, 2026

Implementation date

August 1, 2026

Scope

GB/T 47130-2026 is the English-translated version of 牛冠状病毒感染诊断技术.

GB/T 47130-2026 is the Chinese standard "Diagnostic techniques for bovine coronavirus infection". Its scope clause reads: This document describes the clinical diagnosis, sample collection, preservation and transportation, laboratory sample preparation, and virus isolation and culture of bovine coronavirus infection.

Laboratory diagnostic methods include in vitro diagnostics, indirect immunofluorescence assay, fluorescent RT-PCR, and enzyme-linked immunosorbent assay (ELISA).

This document applies to the diagnosis, epidemiological investigation, and quarantine of bovine coronavirus infection. Its clauses include terms and definitions; abbreviations.

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Document preview — GB/T 47130-2026

National Standard of the People's Republic of China

ICS: 11.220
Classification: B 41

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Preface
Introduction
1.Scope
2 Normative References
3.Terms and Definitions
4.Abbreviations
5.Clinical diagnosis
6.Sample collection, preservation, and transportation
7.Laboratory Sample Preparation
8.Virus isolation and culture
9.Indirect immunofluorescence 6.
10.Fluorescent RT-PCR
11.Enzyme-linked immunosorbent assay (ELISA)
12 Comprehensive judgment
Appendix A (Normative) Sample Preparation and Preparation of Virus Isolation Solution
Appendix B (Informative) CPE of normal HCT-8 cells and cells infected with BCoV (see Figure 12)
Appendix C (Standardized) Preparation of Indirect Immunofluorescence Reagents
Appendix D (Informative) Monoclonal Antibody Preparation
Appendix E (Informative) Reference Figure 15 for Interpreting Indirect Immunofluorescence Results
Appendix F (Normative) Preparation of Nucleic Acid Extraction Solutions
Appendix H (Normative) Preparation of Coated Antigens for Enzyme-Linked Immunosorbent Assay
Appendix I (Normative) Preparation of BCoV Standard Positive and Negative Serum
Appendix J (Standard) Preparation of Enzyme-Linked Immunosorbent Assay Solution

1 Scope

This document describes the clinical diagnosis, sample collection, preservation and transportation, laboratory sample preparation, and virus isolation and culture of bovine coronavirus infection.

Laboratory diagnostic methods include in vitro diagnostics, indirect immunofluorescence assay, fluorescent RT-PCR, and enzyme-linked immunosorbent assay (ELISA).

This document applies to the diagnosis, epidemiological investigation, and quarantine of bovine coronavirus infection.

2 Normative references

The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.

For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.

This document.

GB/T 6682 Specifications and test methods for water used in analytical laboratories

NY/T 541 Technical Specifications for Veterinary Diagnostic Sample Collection, Preservation and Transportation

3 Terms and Definitions

This document does not contain any terms or definitions that need to be defined.

4 Abbreviations

The following abbreviations apply to this document.

BCA. Bicinchoninica acid

BHQ1.Blackhole Quencher 1

BSA. Bovine serum albumin

CPE. Cytopathic effect

Ct. Cyclethreshold

DAPI. 4',6-diamidino-2-phenylindole

FAM. Carboxyfluorescein

HAT. Hypoxanthine, Aminopterin, and Thymidine […]

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 24 pages — is available in the English PDF.

Referenced standards

Normative references

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