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GB/T 47063-2026Diagnostic techniques for equine influenza (English PDF)

马流感诊断技术

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

January 28, 2026

Implementation date

August 1, 2026

Scope

GB/T 47063-2026 is the English-translated version of 马流感诊断技术.

GB/T 47063-2026 is the Chinese standard "Diagnostic techniques for equine influenza". Its scope clause reads: This document describes the clinical diagnosis of equine influenza, as well as EIV isolation and identification, EIV antigen capture ELISA, and H3 subtype EIVRT-.

Laboratory diagnostic methods include PCR, H3N8 subtype EIV real-time fluorescent RT-PCR, hemagglutination inhibition test, and one-way radiation hemolysis test.

This document applies to the diagnosis, quarantine, surveillance, and epidemiological investigation of equine influenza. Its clauses include terms and definitions; abbreviations.

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Document preview — GB/T 47063-2026

National Standard of the People's Republic of China

ICS
11.220
Classification
B 41

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • Preface
  • Introduction
  • 1.Scope
  • 2 Normative References
  • 3.Terms and Definitions
  • 4.Abbreviations
  • 5.Biosafety Measures
  • 6.Clinical diagnosis
  • 7.Sample collection, preservation, and transportation
  • 8.Virus isolation and identification
  • 9 AC-ELISA
  • 10 RT-PCR
  • 11 Real-time fluorescent RT-PCR
  • 12 HI test
  • 13 SRH test
  • 14 Comprehensive Judgment
  • Appendix A (Normative) Solution Preparation
  • Appendix B (Informative) Preparation of EIV Monoclonal Antibody Coating Plates and Enzyme-Labeled Antibodies
  • Appendix C (Informative) Primers and Probes
  • Appendix D (Informative) Preparation of RT-PCR Positive Control Plasmid
  • Appendix E (Informative) Preparation of Positive Control Plasmid for Real-Time Fluorescent RT-PCR
  • Appendix F (Normative) Processing of HI Test Antigen and Serum
  • Appendix G (Normative) Preparation of SRH Test Related Solutions

1 Scope

This document describes the clinical diagnosis of equine influenza, as well as EIV isolation and identification, EIV antigen capture ELISA, and H3 subtype EIVRT-.

Laboratory diagnostic methods include PCR, H3N8 subtype EIV real-time fluorescent RT-PCR, hemagglutination inhibition test, and one-way radiation hemolysis test.

This document applies to the diagnosis, quarantine, surveillance, and epidemiological investigation of equine influenza.

2 Normative references

The contents of the following documents, through normative references within the text, constitute essential provisions of this document. Dated citations are not included.

For references to documents, only the version corresponding to that date applies to this document; for undated references, the latest version (including all amendments) applies.

This document.

GB/T 6682 Specifications and test methods for water used in analytical laboratories

NY/T 541 Technical Specifications for Veterinary Diagnostic Sample Collection, Preservation and Transportation

3 Terms and Definitions

This document does not contain any terms or definitions that need to be defined.

4 Abbreviations

The following abbreviations apply to this document.

CPE. Cytopathic Effects

EI. Equine Influenza

HA. Haemagglutination

MDCK. Madin-DarbyCanineKidney

OD. Optical Density

RNA. Ribonucleic acid (RibonucleicAcid) […]

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 24 pages — is available in the English PDF.

Referenced standards

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GB/T 47063-2026

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